{"id":5097,"date":"2024-01-15T12:23:02","date_gmt":"2024-01-15T12:23:02","guid":{"rendered":"https:\/\/farlong.com\/?p=5097"},"modified":"2024-01-27T10:21:00","modified_gmt":"2024-01-27T10:21:00","slug":"optimization-of-quercitrin-and-total-flavonoids-extraction-from-herba-polygoni-capitati-by-response-surface-methodology","status":"publish","type":"post","link":"https:\/\/directcm.com\/vi\/optimization-of-quercitrin-and-total-flavonoids-extraction-from-herba-polygoni-capitati-by-response-surface-methodology\/","title":{"rendered":"T\u1ed1i \u01b0u h\u00f3a qu\u00e1 tr\u00ecnh chi\u1ebft xu\u1ea5t quercitrin v\u00e0 t\u1ed5ng s\u1ed1 flavonoid t\u1eeb Herba Polygoni Capitati b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p b\u1ec1 m\u1eb7t \u0111\u00e1p \u1ee9ng"},"content":{"rendered":"
\n

tr\u1eebu t\u01b0\u1ee3ng<\/h2>\n
\n
\n

Kh\u00e1ch quan:<\/h3>\n

 <\/p>\n

\u0110\u1ec3 t\u1ed1i \u01b0u h\u00f3a c\u00e1c \u0111i\u1ec1u ki\u1ec7n chi\u1ebft quercitrin v\u00e0 flavonoid t\u1ed5ng s\u1ed1 (TF) t\u1eeb Herba Polygoni Capitati (Touhualiao trong ti\u1ebfng Trung) b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p b\u1ec1 m\u1eb7t ph\u1ea3n \u1ee9ng (RSM).<\/p>\n<\/div>\n

\n

Nguy\u00ean li\u1ec7u v\u00e0 ph\u01b0\u01a1ng ph\u00e1p:<\/h3>\n

 <\/p>\n

Thi\u1ebft k\u1ebf h\u1ed7n h\u1ee3p trung t\u00e2m (CCD) \u0111\u00e3 \u0111\u01b0\u1ee3c \u00e1p d\u1ee5ng \u0111\u1ec3 nghi\u00ean c\u1ee9u t\u00e1c \u0111\u1ed9ng c\u1ee7a ba bi\u1ebfn s\u1ed1 \u0111\u1ed9c l\u1eadp bao g\u1ed3m th\u00e0nh ph\u1ea7n dung m\u00f4i (%), t\u1ef7 l\u1ec7 dung m\u00f4i-v\u1eadt li\u1ec7u (ml\/g) v\u00e0 th\u1eddi gian chi\u1ebft (ph\u00fat) \u0111\u1ed1i v\u1edbi ph\u1ea3n \u1ee9ng, hi\u1ec7u su\u1ea5t quercitrin v\u00e0 TF.<\/p>\n<\/div>\n

\n

K\u1ebft qu\u1ea3:<\/h3>\n

 <\/p>\n

C\u00e1c \u0111i\u1ec1u ki\u1ec7n chi\u1ebft t\u1ed1i \u01b0u nh\u01b0 sau: N\u1ed3ng \u0111\u1ed9 Ethanol, 65,63%; t\u1ef7 l\u1ec7 dung m\u00f4i-v\u1eadt li\u1ec7u, 10,55:1 (ml\/g); th\u1eddi gian chi\u1ebft 54,33 ph\u00fat. M\u00f4 h\u00ecnh to\u00e1n h\u1ecdc \u0111\u00e3 \u0111\u01b0\u1ee3c thi\u1ebft l\u1eadp \u0111\u00e3 m\u00f4 t\u1ea3 t\u1ed1t c\u00e1c y\u1ebfu t\u1ed1 c\u1ee7a c\u00e1c th\u00f4ng s\u1ed1 th\u00ed nghi\u1ec7m v\u00e0 \u0111\u01b0a ra d\u1ef1 \u0111o\u00e1n ch\u00ednh x\u00e1c v\u1ec1 m\u1eb7t th\u1ed1ng k\u00ea v\u1ec1 hi\u1ec7u su\u1ea5t t\u1ed1i \u01b0u c\u1ee7a quercitrin v\u00e0 TF.<\/p>\n<\/div>\n

\n

Ph\u1ea7n k\u1ebft lu\u1eadn:<\/h3>\n

 <\/p>\n

C\u00e1c gi\u00e1 tr\u1ecb th\u1eed nghi\u1ec7m ph\u00f9 h\u1ee3p v\u1edbi nh\u1eefng gi\u00e1 tr\u1ecb \u0111\u01b0\u1ee3c d\u1ef1 \u0111o\u00e1n b\u1edfi m\u00f4 h\u00ecnh to\u00e1n h\u1ecdc \u0111\u00e3 thi\u1ebft l\u1eadp, do \u0111\u00f3 cho th\u1ea5y s\u1ef1 ph\u00f9 h\u1ee3p c\u1ee7a m\u00f4 h\u00ecnh \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng v\u00e0 s\u1ef1 th\u00e0nh c\u00f4ng c\u1ee7a RSM trong vi\u1ec7c t\u1ed1i \u01b0u h\u00f3a c\u00e1c \u0111i\u1ec1u ki\u1ec7n chi\u1ebft.<\/p>\n<\/div>\n<\/div>\n

T\u1eeb kh\u00f3a:\u00a0<\/strong>Thi\u1ebft k\u1ebf h\u1ed7n h\u1ee3p trung t\u00e2m, Khai th\u00e1c, Herba Polygoni Capitati, T\u1ed1i \u01b0u h\u00f3a, Ph\u01b0\u01a1ng ph\u00e1p b\u1ec1 m\u1eb7t \u0111\u00e1p \u1ee9ng<\/span><\/div>\n
<\/div>\n<\/div>\n
\n
\u0110i \u0111\u1ebfn:<\/a><\/div>\n

GI\u1edaI THI\u1ec6U<\/h2>\n

 <\/p>\n

Herba Polygoni Capitati, ph\u1ea7n tr\u00ean m\u1eb7t \u0111\u1ea5t ho\u1eb7c to\u00e0n b\u1ed9 c\u00e2y\u00a0\u0110a gi\u00e1c \u0111\u1ea7u ng\u01b0\u1eddi<\/em>\u00a0H\u1ed9i tr\u01b0\u1edfng-Ham. ex D. Don, \u0111\u00e3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng nh\u01b0 m\u1ed9t trong nh\u1eefng lo\u1ea1i thu\u1ed1c c\u1ed5 truy\u1ec1n Trung Qu\u1ed1c (TCM) t\u1eeb l\u00e2u \u1edf Trung Qu\u1ed1c, \u0111\u1eb7c bi\u1ec7t l\u00e0 \u1edf m\u1ed9t s\u1ed1 v\u00f9ng d\u00e2n t\u1ed9c thi\u1ec3u s\u1ed1. C\u00e1c th\u00e0nh ph\u1ea7n h\u00f3a h\u1ecdc ho\u1ea1t \u0111\u1ed9ng c\u1ee7a Herba Polygoni Capitati \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh l\u00e0 flavonoid, axit phenolic v\u00e0 tannin,[1<\/a>,2<\/a>,3<\/a>] trong \u0111\u00f3 flavonoid \u0111\u01b0\u1ee3c coi l\u00e0 h\u1ee3p ch\u1ea5t c\u00f3 ho\u1ea1t t\u00ednh sinh h\u1ecdc ch\u00ednh. C\u00e1c nghi\u00ean c\u1ee9u d\u01b0\u1ee3c l\u00fd v\u00e0 l\u00e2m s\u00e0ng ch\u1ec9 ra r\u1eb1ng quercetin v\u00e0 c\u00e1c glucoside c\u1ee7a n\u00f3 l\u00e0 c\u00e1c h\u1ee3p ch\u1ea5t c\u00f3 ho\u1ea1t t\u00ednh sinh h\u1ecdc c\u00f3 kh\u1ea3 n\u0103ng ch\u1ed1ng ung th\u01b0,[4<\/a>,5<\/a>] ch\u1ed1ng oxy h\u00f3a [6<\/a>,7<\/a>] v\u00e0 c\u00e1c ho\u1ea1t \u0111\u1ed9ng \u0111i\u1ec1u bi\u1ebfn enzym.[8<\/a>,9<\/a>,10<\/a>] C\u00e1c nghi\u00ean c\u1ee9u d\u1ecbch t\u1ec5 h\u1ecdc c\u0169ng cho th\u1ea5y m\u1ed1i quan h\u1ec7 ngh\u1ecbch \u0111\u1ea3o gi\u1eefa vi\u1ec7c h\u1ea5p th\u1ee5 nhi\u1ec1u flavonoid (ch\u1ee7 y\u1ebfu l\u00e0 quercetin) v\u00e0 b\u1ec7nh tim m\u1ea1ch.[11<\/a>]<\/p>\n

Chi\u1ebft xu\u1ea5t c\u1ee7a TCM \u0111\u00e3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng trong c\u00e1c ph\u01b0\u01a1ng ph\u00e1p ch\u1eefa b\u1ec7nh truy\u1ec1n th\u1ed1ng v\u00e0 c\u00e1c ph\u01b0\u01a1ng thu\u1ed1c th\u1ea3o d\u01b0\u1ee3c trong nhi\u1ec1u th\u1ebf k\u1ef7 tr\u00ean kh\u1eafp th\u1ebf gi\u1edbi[12<\/a>,13<\/a>] v\u00e0 r\u1ea5t nhi\u1ec1u kinh nghi\u1ec7m qu\u00fd b\u00e1u \u0111\u00e3 \u0111\u01b0\u1ee3c t\u00edch l\u0169y trong h\u1ec7 th\u1ed1ng tr\u1ecb li\u1ec7u n\u00e0y. Ephedrine, m\u1ed9t ch\u1ea5t k\u00edch th\u00edch gi\u1ed1ng amphetamine d\u00f9ng l\u00e0m thu\u1ed1c th\u00f4ng m\u0169i cho b\u1ec7nh hen suy\u1ec5n, \u0111\u01b0\u1ee3c chi\u1ebft xu\u1ea5t v\u00e0 ph\u00e2n l\u1eadp t\u1eeb d\u01b0\u1ee3c li\u1ec7u Trung Qu\u1ed1c\u00a0C\u00e2y ma ho\u00e0ng<\/em>.[14<\/a>] Chi\u1ebft xu\u1ea5t c\u1ee7a\u00a0b\u1ea1ch qu\u1ea3<\/em>, \u0111\u1eb7c bi\u1ec7t l\u00e0 EGb 761 \u0111\u01b0\u1ee3c ti\u00eau chu\u1ea9n h\u00f3a l\u00e0 m\u1ed9t trong nh\u1eefng ph\u01b0\u01a1ng thu\u1ed1c th\u1ea3o d\u01b0\u1ee3c \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng r\u1ed9ng r\u00e3i nh\u1ea5t cho ch\u1ee9ng m\u1ea5t tr\u00ed nh\u1edb v\u00e0 suy gi\u1ea3m nh\u1eadn th\u1ee9c.[15<\/a>] V\u00ec nhi\u1ec1u y\u1ebfu t\u1ed1 nh\u01b0 th\u00e0nh ph\u1ea7n dung m\u00f4i, th\u1eddi gian chi\u1ebft, nhi\u1ec7t \u0111\u1ed9 chi\u1ebft, t\u1ef7 l\u1ec7 dung m\u00f4i-nguy\u00ean li\u1ec7u c\u00f3 th\u1ec3 \u1ea3nh h\u01b0\u1edfng \u0111\u00e1ng k\u1ec3 \u0111\u1ebfn hi\u1ec7u qu\u1ea3 chi\u1ebft xu\u1ea5t n\u00ean c\u00e1c th\u00f4ng s\u1ed1 chi\u1ebft xu\u1ea5t c\u1ea7n ph\u1ea3i \u0111\u01b0\u1ee3c t\u1ed1i \u01b0u h\u00f3a \u0111\u1ec3 thu \u0111\u01b0\u1ee3c nhi\u1ec1u th\u00e0nh ph\u1ea7n c\u00f3 gi\u00e1 tr\u1ecb ph\u00f2ng b\u1ec7nh ho\u1eb7c \u0111i\u1ec1u tr\u1ecb h\u01a1n cho con ng\u01b0\u1eddi.[16<\/a>]<\/p>\n

Trong s\u1ed1 t\u1ea5t c\u1ea3 c\u00e1c h\u1ee3p ch\u1ea5t \u0111\u01b0\u1ee3c tr\u00ecnh b\u00e0y trong Herba Polygoni Capitati, axit phenolic v\u00e0 th\u00e0nh ph\u1ea7n flavonoid \u0111\u00e3 nh\u1eadn \u0111\u01b0\u1ee3c nhi\u1ec1u s\u1ef1 ch\u00fa \u00fd nh\u1ea5t v\u00e0 \u0111\u01b0\u1ee3c coi l\u00e0 c\u00f3 t\u1ea7m quan tr\u1ecdng \u0111\u1ed1i v\u1edbi t\u00e1c d\u1ee5ng d\u01b0\u1ee3c l\u00fd c\u1ee7a ch\u00fang.[17<\/a>] Vi\u1ec7c chi\u1ebft xu\u1ea5t axit Gallic (GA) ho\u1eb7c TF t\u1eeb Herba Polygoni Capitati ch\u1ec9 theo ph\u01b0\u01a1ng ph\u00e1p ti\u1ebfp c\u1eadn m\u1ed9t y\u1ebfu t\u1ed1 t\u1ea1i m\u1ed9t th\u1eddi \u0111i\u1ec3m,[18<\/a>] ho\u1eb7c li\u00ean quan \u0111\u1ebfn thi\u1ebft k\u1ebf tr\u1ef1c giao,[19<\/a>] \u0111\u00e3 \u0111\u01b0\u1ee3c b\u00e1o c\u00e1o tr\u01b0\u1edbc \u0111\u00f3. C\u00e1ch ti\u1ebfp c\u1eadn m\u1ed9t y\u1ebfu t\u1ed1 t\u1ea1i m\u1ed9t th\u1eddi \u0111i\u1ec3m, trong \u0111\u00f3 t\u1ea1i m\u1ed9t th\u1eddi \u0111i\u1ec3m ch\u1ec9 c\u00f3 m\u1ed9t y\u1ebfu t\u1ed1 c\u00f3 th\u1ec3 thay \u0111\u1ed5i trong khi gi\u1eef nguy\u00ean t\u1ea5t c\u1ea3 c\u00e1c y\u1ebfu t\u1ed1 kh\u00e1c,[20<\/a>] t\u1ed1n th\u1eddi gian v\u00e0 c\u00f3 th\u1ec3 \u0111\u01b0a ra k\u1ebft lu\u1eadn sai l\u1ec7ch v\u00ec n\u00f3 kh\u00f4ng bao g\u1ed3m c\u00e1c t\u00e1c \u0111\u1ed9ng t\u01b0\u01a1ng t\u00e1c gi\u1eefa c\u00e1c y\u1ebfu t\u1ed1. Thi\u1ebft k\u1ebf tr\u1ef1c giao r\u1ea5t h\u1eefu \u00edch trong thi\u1ebft k\u1ebf th\u1eed nghi\u1ec7m [21<\/a>] nh\u01b0ng n\u00f3 kh\u00f4ng \u0111\u01b0a ra d\u1ef1 \u0111o\u00e1n t\u1eeb ph\u01b0\u01a1ng tr\u00ecnh m\u00f4 h\u00ecnh. Ph\u01b0\u01a1ng ph\u00e1p b\u1ec1 m\u1eb7t \u0111\u00e1p \u1ee9ng (RSM), m\u1ed9t t\u1eadp h\u1ee3p c\u00e1c k\u1ef9 thu\u1eadt to\u00e1n h\u1ecdc v\u00e0 th\u1ed1ng k\u00ea, c\u00f3 th\u1ec3 kh\u1eafc ph\u1ee5c nh\u1eefng nh\u01b0\u1ee3c \u0111i\u1ec3m n\u00e0y v\u00ec n\u00f3 t\u00ednh \u0111\u1ebfn c\u00e1c hi\u1ec7u \u1ee9ng t\u01b0\u01a1ng t\u00e1c c\u00f3 th\u1ec3 c\u00f3 gi\u1eefa c\u00e1c bi\u1ebfn.[22<\/a>,23<\/a>] Bi\u1ec3u \u0111\u1ed3 b\u1ec1 m\u1eb7t ph\u1ea3n h\u1ed3i v\u00e0 bi\u1ec3u \u0111\u1ed3 \u0111\u01b0\u1eddng vi\u1ec1n c\u1ee7a ph\u1ea3n h\u1ed3i d\u01b0\u1edbi d\u1ea1ng h\u00e0m c\u1ee7a c\u00e1c tham s\u1ed1 \u0111\u1ed9c l\u1eadp c\u00f3 th\u1ec3 thu \u0111\u01b0\u1ee3c b\u1eb1ng RSM.[24<\/a>] Trong nghi\u00ean c\u1ee9u hi\u1ec7n t\u1ea1i, ph\u01b0\u01a1ng ph\u00e1p RSM \u0111\u00e3 \u0111\u01b0\u1ee3c \u00e1p d\u1ee5ng \u0111\u1ec3 t\u1ed1i \u01b0u h\u00f3a c\u00e1c \u0111i\u1ec1u ki\u1ec7n chi\u1ebft (t\u1ef7 l\u1ec7 dung m\u00f4i, t\u1ef7 l\u1ec7 dung m\u00f4i-nguy\u00ean li\u1ec7u, th\u1eddi gian chi\u1ebft, v.v.) t\u1eeb Herba Polygoni Capitati nh\u1eb1m t\u1ed1i \u0111a h\u00f3a hi\u1ec7u su\u1ea5t c\u1ee7a quercitrin v\u00e0 TF \u0111\u1ed3ng th\u1eddi.<\/p>\n

 <\/p>\n<\/div>\n

\n
\u0110i \u0111\u1ebfn:<\/a><\/div>\n

NGUY\u00caN LI\u1ec6U V\u00c0 PH\u01af\u01a0NG PH\u00c1P<\/h2>\n

 <\/p>\n

\n

V\u1eadt li\u1ec7u v\u00e0 h\u00f3a ch\u1ea5t<\/h3>\n

 <\/p>\n

Herba Polygoni Capitati, \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh v\u1ec1 m\u1eb7t th\u1ef1c v\u1eadt h\u1ecdc b\u1edfi gi\u00e1o s\u01b0 Deyuan Chen, \u0111\u01b0\u1ee3c l\u1ea5y t\u1eeb T\u1eadp \u0111o\u00e0n D\u01b0\u1ee3c ph\u1ea9m Guizhou Warmen, Qu\u00fd Ch\u00e2u, Trung Qu\u1ed1c. To\u00e0n b\u1ed9 c\u00e2y Herba Polygoni Capitati \u0111\u01b0\u1ee3c s\u1ea5y kh\u00f4 trong l\u00f2 \u0111\u1ed1i l\u01b0u c\u01b0\u1ee1ng b\u1ee9c (101-2AB Taijin, Trung Qu\u1ed1c) \u1edf 50\u00b0C trong 4 gi\u1edd. M\u1eabu kh\u00f4 \u0111\u01b0\u1ee3c nghi\u1ec1n trong m\u00e1y nghi\u1ec1n quay (DFY-200 Wenling, Trung Qu\u1ed1c) v\u00e0 b\u1ed9t \u0111\u01b0\u1ee3c s\u00e0ng. C\u00e1c h\u1ea1t c\u00f3 k\u00edch th\u01b0\u1edbc t\u1eeb 10 \u0111\u1ebfn 40 m\u1eaft l\u01b0\u1edbi (0,3~2mm, id) \u0111\u00e3 \u0111\u01b0\u1ee3c thu th\u1eadp \u0111\u1ec3 nghi\u00ean c\u1ee9u. C\u00e1c h\u1ee3p ch\u1ea5t tham chi\u1ebfu quercitrin (111538-200403) v\u00e0 rutin (080-9303) \u0111\u01b0\u1ee3c mua t\u1eeb Vi\u1ec7n Ki\u1ec3m so\u00e1t S\u1ea3n ph\u1ea9m Sinh h\u1ecdc v\u00e0 D\u01b0\u1ee3c ph\u1ea9m Qu\u1ed1c gia Trung Qu\u1ed1c (B\u1eafc Kinh, Trung Qu\u1ed1c). Acetonitril v\u00e0 metanol l\u00e0 lo\u1ea1i HPLC (Tedia, Hoa K\u1ef3). N\u01b0\u1edbc l\u00e0 n\u01b0\u1edbc u\u1ed1ng tinh khi\u1ebft Robust (C\u00f4ng ty TNHH Th\u1ef1c ph\u1ea9m v\u00e0 \u0110\u1ed3 u\u1ed1ng L\u00ea Bai Shi, Qu\u1ea3ng \u0110\u00f4ng). Axit formic lo\u1ea1i MS (Roe Scientific Inc, Hoa K\u1ef3). T\u1ea5t c\u1ea3 c\u00e1c h\u00f3a ch\u1ea5t v\u00e0 dung m\u00f4i kh\u00e1c \u0111\u1ec1u thu\u1ed9c lo\u1ea1i ph\u00e2n t\u00edch v\u00e0 c\u00f3 s\u1eb5n tr\u00ean th\u1ecb tr\u01b0\u1eddng.<\/p>\n

 <\/p>\n<\/div>\n

\n

Chu\u1ea9n b\u1ecb m\u1eabu<\/h3>\n

 <\/p>\n

B\u1ed9t Herba Polygoni Capitati 2g \u0111\u01b0\u1ee3c c\u00e2n ch\u00ednh x\u00e1c v\u00e0 chi\u1ebft xu\u1ea5t trong nh\u1eefng \u0111i\u1ec1u ki\u1ec7n nh\u1ea5t \u0111\u1ecbnh, sau \u0111\u00f3 d\u1ecbch chi\u1ebft \u0111\u01b0\u1ee3c ly t\u00e2m \u1edf 3500\u00a0v\u00f2ng\/ph\u00fat<\/em>\u00a0trong 10 ph\u00fat. Ph\u1ea7n n\u1ed5i ph\u00eda tr\u00ean \u0111\u01b0\u1ee3c thu th\u1eadp v\u00e0 l\u1ecdc qua gi\u1ea5y l\u1ecdc \u0111\u1ec3 ph\u00e2n t\u00edch ti\u1ebfp theo.<\/p>\n

 <\/p>\n<\/div>\n

\n

Thi\u1ebft b\u1ecb<\/h3>\n

 <\/p>\n

C\u00e1c h\u1ec7 th\u1ed1ng ph\u00e2n t\u00edch \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng trong nghi\u00ean c\u1ee9u n\u00e0y nh\u01b0 sau: Ph\u1ed5 UV-Vis \u0111\u01b0\u1ee3c ghi l\u1ea1i tr\u00ean m\u00e1y quang ph\u1ed5 ch\u00f9m tia k\u00e9p (Cary 100-Varian) v\u1edbi c\u00e1c cuvet c\u00f3 chi\u1ec1u d\u00e0i \u0111\u01b0\u1eddng truy\u1ec1n 1 cm. H\u1ec7 th\u1ed1ng UHPLC-DAD d\u00f2ng DIONEX\/UltiMate 3000 bao g\u1ed3m b\u1ed9 kh\u1eed kh\u00ed ch\u00e2n kh\u00f4ng, b\u01a1m b\u1eadc b\u1ed1n, b\u1ed9 l\u1ea5y m\u1eabu t\u1ef1 \u0111\u1ed9ng, ng\u0103n c\u1ed9t \u1ed5n \u0111\u1ecbnh nhi\u1ec7t v\u00e0 m\u00e1y d\u00f2 DAD (DIONEX, ThermoFisher, Sunnyvale, USA) \u0111\u00e3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 thu s\u1eafc k\u00fd \u0111\u1ed3.<\/p>\n

 <\/p>\n<\/div>\n

\n

X\u00e1c \u0111\u1ecbnh h\u00e0m l\u01b0\u1ee3ng flavonoid t\u1ed5ng s\u1ed1<\/h3>\n

 <\/p>\n

T\u1ed5ng h\u00e0m l\u01b0\u1ee3ng flavonoid (TFC) \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh theo ph\u01b0\u01a1ng ph\u00e1p so m\u00e0u nh\u00f4m \u0111\u01b0\u1ee3c m\u00f4 t\u1ea3 tr\u01b0\u1edbc \u0111\u00e2y [25<\/a>,26<\/a>,27<\/a>,28<\/a>] v\u1edbi nh\u1eefng s\u1eeda \u0111\u1ed5i. T\u00f3m l\u1ea1i, m\u1ed9t ph\u1ea7n d\u1ecbch (0,5ml) c\u1ee7a m\u1ed7i chi\u1ebft xu\u1ea5t th\u1ef1c v\u1eadt \u0111\u01b0\u1ee3c tr\u1ed9n v\u1edbi 4,3ml dung m\u00f4i li\u00ean quan, sau \u0111\u00f3 th\u00eam 0,1ml nh\u00f4m clorua 10% (AlCl3<\/sub>) v\u00e0 0,1ml natri axetat 1M (CH3<\/sub>\u00a0COONa). Rutin \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng l\u00e0m ti\u00eau chu\u1ea9n tham kh\u1ea3o. Sau khi \u1ee7 \u1edf nhi\u1ec7t \u0111\u1ed9 ph\u00f2ng trong 30 ph\u00fat, h\u1ed7n h\u1ee3p ph\u1ea3n \u1ee9ng \u0111\u01b0\u1ee3c \u0111o \u1edf b\u01b0\u1edbc s\u00f3ng 405nm tr\u00ean m\u1eabu tr\u1eafng. Gi\u00e1 tr\u1ecb TFC c\u1ee7a m\u1ed7i m\u1eabu \u0111\u01b0\u1ee3c t\u00ednh to\u00e1n v\u00e0 bi\u1ec3u th\u1ecb b\u1eb1ng miligam rutin t\u01b0\u01a1ng \u0111\u01b0\u01a1ng tr\u00ean gam v\u1eadt li\u1ec7u kh\u00f4 (DM).<\/p>\n

 <\/p>\n<\/div>\n

\n

T\u00e1ch s\u1eafc k\u00fd<\/h3>\n

 <\/p>\n

Ph\u00e2n t\u00edch HPLC c\u1ee7a d\u1ecbch chi\u1ebft \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng d\u00f2ng DIONEX\/UltiMate 3000. Vi\u1ec7c ph\u00e2n t\u00e1ch \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n tr\u00ean ZORBAX SB-C18<\/sub>\u00a0c\u1ed9t (150 mm \u00d7 4,6 mm, 5 \u03bcm; Agilent, CA, USA) v\u1edbi t\u1ed1c \u0111\u1ed9 d\u00f2ng 1,0ml\/ph\u00fat. Pha \u0111\u1ed9ng bao g\u1ed3m s\u1ef1 k\u1ebft h\u1ee3p c\u1ee7a A (acetonitril) v\u00e0 B (axit formic 0,2% trong n\u01b0\u1edbc). \u0110\u1ed9 d\u1ed1c tuy\u1ebfn t\u00ednh l\u00e0 t\u1eeb 5% \u0111\u1ebfn 15% A (v\/v<\/em>) sau 5 ph\u00fat, \u0111\u1ebfn 16% A sau 10 ph\u00fat, \u0111\u1ebfn 20% A sau 20 ph\u00fat v\u00e0 sau \u0111\u00f3 \u0111\u01b0\u1ee3c gi\u1eef \u1edf 20% A sau 22 ph\u00fat. M\u1ed7i l\u1ea7n ch\u1ea1y \u0111\u01b0\u1ee3c theo sau b\u1edfi th\u1eddi gian c\u00e2n b\u1eb1ng l\u00e0 5 ph\u00fat. B\u01b0\u1edbc s\u00f3ng c\u1ee7a detector UV \u0111\u01b0\u1ee3c \u0111\u1eb7t \u1edf 260 nm v\u00e0 nhi\u1ec7t \u0111\u1ed9 c\u1ee7a l\u00f2 c\u1ed9t \u0111\u01b0\u1ee3c duy tr\u00ec \u1edf 30\u00b0C. C\u00e1c m\u1eabu \u0111\u01b0\u1ee3c l\u1ecdc tr\u01b0\u1edbc khi ti\u00eam (b\u1ed9 l\u1ecdc \u1ed1ng ti\u00eam PTFE; 0,45 \u03bcm; Hanbon Ltd, Trung Qu\u1ed1c; 013045). S\u1ed1 li\u1ec7u \u0111\u01b0\u1ee3c thu th\u1eadp v\u00e0 ph\u00e2n t\u00edch b\u1eb1ng ph\u1ea7n m\u1ec1m Chromeleon 7.1.<\/p>\n

 <\/p>\n<\/div>\n

\n

Chu\u1ea9n b\u1ecb c\u00e1c dung d\u1ecbch chu\u1ea9n<\/h3>\n

 <\/p>\n

Dung d\u1ecbch chu\u1ea9n g\u1ed1c c\u1ee7a rutin v\u00e0 quercitrin \u0111\u01b0\u1ee3c chu\u1ea9n b\u1ecb trong metanol, \u1edf n\u1ed3ng \u0111\u1ed9 l\u1ea7n l\u01b0\u1ee3t l\u00e0 190,8 \u03bcg\/ml v\u00e0 106,0 \u03bcg\/ml. \u0110\u01b0\u1eddng cong chu\u1ea9n c\u1ee7a quercitrin v\u00e0 TF \u0111\u01b0\u1ee3c hi\u1ec7u ch\u1ec9nh b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng ph\u01b0\u01a1ng tr\u00ecnh h\u1ed3i quy b\u00ecnh ph\u01b0\u01a1ng nh\u1ecf nh\u1ea5t tuy\u1ebfn t\u00ednh r\u00fat ra t\u1eeb di\u1ec7n t\u00edch pic v\u00e0 \u0111\u1ed9 h\u1ea5p th\u1ee5 t\u01b0\u01a1ng \u1ee9ng, n\u1ed3ng \u0111\u1ed9 quercitrin v\u00e0 TF trong c\u00e1c m\u1eabu \u0111\u01b0\u1ee3c t\u00ednh theo \u0111\u01b0\u1eddng cong chu\u1ea9n.<\/p>\n

 <\/p>\n<\/div>\n

\n

L\u1ef1a ch\u1ecdn \u0111i\u1ec1u ki\u1ec7n chi\u1ebft th\u00edch h\u1ee3p<\/h3>\n

 <\/p>\n

Tr\u01b0\u1edbc khi ph\u00e1t tri\u1ec3n nghi\u00ean c\u1ee9u b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng RSM, m\u1ed9t lo\u1ea1t c\u00e1c th\u1eed nghi\u1ec7m s\u01a1 b\u1ed9 \u0111\u00e3 \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n \u0111\u1ec3 ch\u1ecdn c\u00e1c bi\u1ebfn \u0111\u1ed9c l\u1eadp c\u00f3 li\u00ean quan v\u00e0 x\u00e1c \u0111\u1ecbnh ph\u1ea1m vi th\u1eed nghi\u1ec7m c\u1ee7a ch\u00fang. B\u01b0\u1edbc \u0111\u1ea7u ti\u00ean c\u1ee7a th\u00ed nghi\u1ec7m s\u01a1 b\u1ed9 l\u00e0 l\u1ef1a ch\u1ecdn ph\u01b0\u01a1ng ph\u00e1p th\u00edch h\u1ee3p \u0111\u1ec3 chi\u1ebft xu\u1ea5t Herba Polygoni Capitati. Ba ph\u01b0\u01a1ng ph\u00e1p kh\u00e1c nhau, c\u1ee5 th\u1ec3 l\u00e0 ng\u00e2m, chi\u1ebft h\u1ed3i l\u01b0u v\u00e0 chi\u1ebft si\u00eau \u00e2m \u0111\u00e3 \u0111\u01b0\u1ee3c th\u1eed nghi\u1ec7m. B\u01b0\u1edbc th\u1ee9 hai c\u1ee7a th\u00ed nghi\u1ec7m s\u01a1 b\u1ed9 l\u00e0 l\u1ef1a ch\u1ecdn dung m\u00f4i chi\u1ebft th\u00edch h\u1ee3p cho Herba Polygoni Capitati. S\u1eed d\u1ee5ng m\u1ed9t lo\u1ea1t c\u00e1c dung m\u00f4i chi\u1ebft kh\u00e1c nhau trong kho\u1ea3ng 0-100% (etanol ho\u1eb7c metanol trong n\u01b0\u1edbc), dung m\u00f4i t\u1ed1t nh\u1ea5t \u0111\u00e3 \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh. Th\u1ee9 ba, h\u1ec7 s\u1ed1 t\u1ef7 l\u1ec7 dung m\u00f4i - v\u1eadt li\u1ec7u (6:1, 8:1, 10:1, 12:1, 14:1;\u00a0v\/m<\/em>) khi chi\u1ebft \u0111\u01b0\u1ee3c kh\u1ea3o s\u00e1t b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng etanol: n\u01b0\u1edbc (70:30,\u00a0v\/v<\/em>) l\u00e0m dung m\u00f4i \u1edf \u0111i\u1ec1u ki\u1ec7n chi\u1ebft si\u00eau \u00e2m trong 30 ph\u00fat \u1edf nhi\u1ec7t \u0111\u1ed9 ph\u00f2ng. Th\u1ee9 t\u01b0, k\u00edch th\u01b0\u1edbc h\u1ea1t th\u00edch h\u1ee3p \u0111\u00e3 \u0111\u01b0\u1ee3c ch\u1ecdn \u0111\u1ec3 nghi\u00ean c\u1ee9u th\u00eam. Cu\u1ed1i c\u00f9ng, th\u1eddi gian chi\u1ebft (10 ph\u00fat, 20 ph\u00fat, 30 ph\u00fat, 40 ph\u00fat, 50 ph\u00fat, 60 ph\u00fat) c\u0169ng \u0111\u00e3 \u0111\u01b0\u1ee3c th\u1eed nghi\u1ec7m.<\/p>\n

D\u1ef1a tr\u00ean nh\u1eefng k\u1ebft qu\u1ea3 n\u00e0y, ba c\u1ea5p \u0111\u1ed9 (d\u01b0\u1edbi, gi\u1eefa, tr\u00ean) c\u1ee7a t\u1eebng bi\u1ebfn li\u00ean quan \u0111\u00e3 \u0111\u01b0\u1ee3c ch\u1ecdn cho thi\u1ebft k\u1ebf t\u1ed5ng h\u1ee3p trung t\u00e2m (CCD) \u0111\u1ec3 ph\u00e2n t\u00edch m\u1eabu ph\u1ea3n h\u1ed3i v\u00e0 thi\u1ebft l\u1eadp m\u00f4 h\u00ecnh.<\/p>\n

 <\/p>\n<\/div>\n

\n

Ph\u00e2n t\u00edch th\u1ed1ng k\u00ea<\/h3>\n

 <\/p>\n

D\u1eef li\u1ec7u th\u1ef1c nghi\u1ec7m thu \u0111\u01b0\u1ee3c t\u1eeb quy tr\u00ecnh CCD \u0111\u01b0\u1ee3c ph\u00e2n t\u00edch b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng RSM \u0111\u1ec3 ph\u00f9 h\u1ee3p v\u1edbi m\u00f4 h\u00ecnh \u0111a th\u1ee9c b\u1eadc hai sau (Ph\u01b0\u01a1ng tr\u00ecnh (1) v\u00e0 c\u00e1c h\u1ec7 s\u1ed1 h\u1ed3i quy thu \u0111\u01b0\u1ee3c.<\/p>\n

\"An<\/p>\n

\u1ede \u0111\u00e2u X1<\/sub>, X2<\/sub>,\u2026, Xk<\/sub>\u00a0l\u00e0 c\u00e1c bi\u1ebfn \u0111\u1ed9c l\u1eadp \u0111\u01b0\u1ee3c m\u00e3 h\u00f3a \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn ph\u1ea3n h\u1ed3i Y; \u03b20<\/sub>, \u03b2T\u00f4i<\/sub>\u00a0(i = 1, 2,\u2026, k), \u03b2ii<\/sub>\u00a0(i = 1, 2,\u2026, k) v\u00e0 \u03b2ij<\/sub>\u00a0(i = 1, 2,\u2026, k) l\u1ea7n l\u01b0\u1ee3t l\u00e0 c\u00e1c h\u1ec7 s\u1ed1 h\u1ed3i quy cho c\u00e1c s\u1ed1 h\u1ea1ng ch\u1eb7n, tuy\u1ebfn t\u00ednh, b\u1eadc hai v\u00e0 t\u01b0\u01a1ng t\u00e1c; k l\u00e0 s\u1ed1 l\u01b0\u1ee3ng bi\u1ebfn.<\/p>\n

Ph\u1ea7n m\u1ec1m Design Expert phi\u00ean b\u1ea3n 8.0.5b (STAT-EASE Inc.) \u0111\u00e3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 h\u1ed3i quy v\u00e0 ph\u00e2n t\u00edch \u0111\u1ed3 h\u1ecda c\u1ee7a d\u1eef li\u1ec7u th\u1eed nghi\u1ec7m. Ch\u1ea5t l\u01b0\u1ee3ng \u0111\u1ed9 ph\u00f9 h\u1ee3p c\u1ee7a m\u00f4 h\u00ecnh \u0111\u01b0\u1ee3c \u0111\u00e1nh gi\u00e1 b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng c\u00e1c h\u1ec7 s\u1ed1 x\u00e1c \u0111\u1ecbnh (R<\/em>2<\/sup>) v\u00e0 ph\u00e2n t\u00edch ph\u01b0\u01a1ng sai (ANOVA). C\u00e1c b\u1ec1 m\u1eb7t \u0111\u00e1p \u1ee9ng v\u00e0 \u0111\u1ed3 th\u1ecb \u0111\u01b0\u1eddng vi\u1ec1n \u0111\u01b0\u1ee3c ph\u00e1t tri\u1ec3n b\u1eb1ng ph\u01b0\u01a1ng tr\u00ecnh \u0111a th\u1ee9c b\u1eadc hai \u0111\u01b0\u1ee3c trang b\u1ecb.<\/p>\n

 <\/p>\n<\/div>\n<\/div>\n

\n
\u0110i \u0111\u1ebfn:<\/a><\/div>\n

K\u1ebeT QU\u1ea2 V\u00c0 TH\u1ea2O LU\u1eacN<\/h2>\n

 <\/p>\n

\n

\u0110\u1ed9 ch\u00ednh x\u00e1c c\u1ee7a h\u1ec7 th\u1ed1ng, \u0111\u1ed9 tuy\u1ebfn t\u00ednh c\u1ee7a HPLC ho\u1eb7c UV-Vis<\/h3>\n

 <\/p>\n

Quercitrin v\u00e0 TF \u0111\u01b0\u1ee3c ch\u1ec9 \u0111\u1ecbnh l\u00e0m ch\u1ea5t \u0111\u00e1nh d\u1ea5u \u0111\u1ec3 \u0111\u00e1nh gi\u00e1 hi\u1ec7u qu\u1ea3 chi\u1ebft. H\u1ed3 s\u01a1 HPLC c\u1ee7a ti\u00eau chu\u1ea9n tham chi\u1ebfu v\u00e0 chi\u1ebft xu\u1ea5t Herba Polygoni Capitati \u0111\u01b0\u1ee3c tr\u00ecnh b\u00e0y trong\u00a0H\u00ecnh 1<\/a>. \u0110\u01b0\u1eddng cong hi\u1ec7u chu\u1ea9n di\u1ec7n t\u00edch pic so v\u1edbi n\u1ed3ng \u0111\u1ed9 quercitrin l\u00e0\u00a0y<\/em>\u00a0= 47.622\u00a0x<\/em>\u00a0\u2212 0,2231 v\u1edbi ph\u1ea1m vi tuy\u1ebfn t\u00ednh t\u1eeb 0,033-1,06 \u03bcg (R<\/em>2<\/sup>\u00a0= 1.0000,\u00a0y<\/em>\u00a0= di\u1ec7n t\u00edch pic v\u00e0\u00a0x<\/em>\u00a0= l\u01b0\u1ee3ng quercitrin). \u0110\u01b0\u1eddng cong hi\u1ec7u chu\u1ea9n c\u1ee7a \u0111\u1ed9 h\u1ea5p th\u1ee5 so v\u1edbi l\u01b0\u1ee3ng TF l\u00e0\u00a0y<\/em>\u00a0= 0.0029\u00a0x<\/em>\u00a0\u2013 0,0088 v\u1edbi ph\u1ea1m vi tuy\u1ebfn t\u00ednh t\u1eeb 74,32-267,12 \u03bcg (R<\/em>2<\/sup>\u00a0= 0.9996,\u00a0y<\/em>\u00a0= \u0111\u1ed9 h\u1ea5p th\u1ee5 v\u00e0\u00a0x<\/em>\u00a0= s\u1ed1 ti\u1ec1n TF).<\/p>\n

\n
\"An<\/div>\n
\n
H\u00ecnh 1<\/a><\/div>\n
\n

S\u1eafc k\u00fd \u0111\u1ed3 HPLC c\u1ee7a h\u1ee3p ch\u1ea5t tham chi\u1ebfu \u0111\u00e3 \u0111\u01b0\u1ee3c x\u00e1c th\u1ef1c c\u1ee7a quercitrin (a) v\u00e0 d\u1ecbch chi\u1ebft Herba Polygoni Capitati (b) \u1edf b\u01b0\u1edbc s\u00f3ng 260nm<\/p>\n

 <\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

\n

X\u00e1c \u0111\u1ecbnh c\u00e1c th\u00f4ng s\u1ed1 v\u00e0 l\u1ef1a ch\u1ecdn c\u00e1c m\u1ee9c<\/h3>\n
\n

 <\/p>\n

\u1ea2nh h\u01b0\u1edfng c\u1ee7a ph\u01b0\u01a1ng ph\u00e1p chi\u1ebft xu\u1ea5t<\/h4>\n

 <\/p>\n

Ba ph\u01b0\u01a1ng ph\u00e1p chi\u1ebft kh\u00e1c nhau, bao g\u1ed3m ng\u00e2m, h\u1ed3i l\u01b0u v\u00e0 chi\u1ebft si\u00eau \u00e2m \u0111\u00e3 \u0111\u01b0\u1ee3c th\u1eed nghi\u1ec7m v\u1edbi c\u00e1c gi\u00e1 tr\u1ecb th\u00f4ng s\u1ed1 sau: k\u00edch th\u01b0\u1edbc h\u1ea1t, 10 m\u1eaft l\u01b0\u1edbi; t\u1ef7 l\u1ec7 dung m\u00f4i-v\u1eadt li\u1ec7u, 10:1 (ml\/g); th\u1eddi gian chi\u1ebft, 30 ph\u00fat \u0111\u1ed1i v\u1edbi chi\u1ebft h\u1ed3i l\u01b0u v\u00e0 chi\u1ebft si\u00eau \u00e2m, 12h \u0111\u1ed1i v\u1edbi chi\u1ebft ng\u00e2m; th\u00e0nh ph\u1ea7n dung m\u00f4i, dung d\u1ecbch ethanol 70%; nhi\u1ec7t \u0111\u1ed9, nhi\u1ec7t \u0111\u1ed9 ph\u00f2ng. L\u01b0\u1ee3ng quercitrin v\u00e0 TF chi\u1ebft \u0111\u01b0\u1ee3c tr\u00ean m\u1ed7i gam DM \u0111\u01b0\u1ee3c tr\u00ecnh b\u00e0y \u1edf\u00a0H\u00ecnh 2<\/a>. ANOVA cho k\u1ebft qu\u1ea3 th\u1eed nghi\u1ec7m cho th\u1ea5y s\u1ef1 kh\u00e1c bi\u1ec7t \u0111\u00e1ng k\u1ec3 gi\u1eefa ba ph\u01b0\u01a1ng ph\u00e1p chi\u1ebft xu\u1ea5t n\u00e0y (P<\/em>\u00a0<0,01). Trong khi \u0111\u00f3, ph\u01b0\u01a1ng ph\u00e1p chi\u1ebft si\u00eau \u00e2m cho hi\u1ec7u su\u1ea5t quercitrin v\u00e0 TF cao nh\u1ea5t n\u00ean \u0111\u01b0\u1ee3c ch\u00fang t\u00f4i l\u1ef1a ch\u1ecdn l\u00e0m ph\u01b0\u01a1ng ph\u00e1p chi\u1ebft.<\/p>\n

\n
\"An<\/div>\n
\n
H\u00ecnh 2<\/a><\/div>\n
\n

\u1ea2nh h\u01b0\u1edfng c\u1ee7a c\u00e1c ph\u01b0\u01a1ng ph\u00e1p chi\u1ebft xu\u1ea5t quercitrin (tr\u00e1i) v\u00e0 TF (ph\u1ea3i) kh\u00e1c nhau t\u1eeb Herba Polygoni Capitati<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

\n

 <\/p>\n

\u1ea2nh h\u01b0\u1edfng c\u1ee7a dung m\u00f4i chi\u1ebft<\/h4>\n

 <\/p>\n

Dung m\u00f4i l\u00e0 y\u1ebfu t\u1ed1 quan tr\u1ecdng \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn \u0111\u1ed9 thu h\u1ed3i ch\u1ea5t ph\u00e2n t\u00edch. Trong nghi\u00ean c\u1ee9u n\u00e0y, n\u01b0\u1edbc, c\u00e1c n\u1ed3ng \u0111\u1ed9 metanol kh\u00e1c nhau v\u00e0 c\u00e1c n\u1ed3ng \u0111\u1ed9 etanol kh\u00e1c nhau \u0111\u00e3 \u0111\u01b0\u1ee3c th\u1eed nghi\u1ec7m. Nh\u01b0 th\u1ec3 hi\u1ec7n trong\u00a0H\u00ecnh 3<\/a>, hi\u1ec7u su\u1ea5t chi\u1ebft c\u1ee7a dung d\u1ecbch ethanol 60% l\u00e0 cao nh\u1ea5t. Do \u0111\u00f3, etanol 50%, 60% v\u00e0 70% \u0111\u01b0\u1ee3c ch\u1ecdn l\u00e0m m\u1ee9c th\u1ea5p h\u01a1n, trung b\u00ecnh v\u00e0 cao h\u01a1n c\u1ee7a th\u00e0nh ph\u1ea7n dung m\u00f4i \u0111\u1ec3 nghi\u00ean c\u1ee9u th\u00eam v\u1ec1 RSM.<\/p>\n

\n
\"An<\/div>\n
\n
H\u00ecnh 3<\/a><\/div>\n
\n

\u1ea2nh h\u01b0\u1edfng c\u1ee7a th\u00e0nh ph\u1ea7n dung m\u00f4i \u0111\u1ebfn qu\u00e1 tr\u00ecnh chi\u1ebft quercitrin v\u00e0 TF t\u1eeb Herba Polygoni Capitati c\u00f3 h\u1ed7 tr\u1ee3 si\u00eau \u00e2m<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

\n

 <\/p>\n

T\u1ef7 l\u1ec7 dung m\u00f4i-v\u1eadt li\u1ec7u<\/h4>\n

 <\/p>\n

\u1ea2nh h\u01b0\u1edfng c\u1ee7a t\u1ef7 l\u1ec7 dung m\u00f4i-v\u1eadt li\u1ec7u \u0111\u1ebfn qu\u00e1 tr\u00ecnh chi\u1ebft \u0111\u01b0\u1ee3c th\u1eed nghi\u1ec7m v\u1edbi n\u0103m gi\u00e1 tr\u1ecb (6:1, 8:1, 10:1, 12:1, 14:1;\u00a0v\/m<\/em>). Gi\u00e1 tr\u1ecb t\u1ef7 l\u1ec7 dung m\u00f4i-v\u1eadt li\u1ec7u ph\u00f9 h\u1ee3p s\u1ebd \u0111\u01b0\u1ee3c l\u1ef1a ch\u1ecdn d\u1ef1a tr\u00ean c\u00e1c quy t\u1eafc [29<\/a>] r\u1eb1ng gi\u00e1 tr\u1ecb kh\u00f4ng \u0111\u01b0\u1ee3c qu\u00e1 nh\u1ecf (c\u00e1c h\u1ee3p ch\u1ea5t c\u00f3 ho\u1ea1t t\u00ednh sinh h\u1ecdc kh\u00f4ng th\u1ec3 \u0111\u01b0\u1ee3c chi\u1ebft xu\u1ea5t ho\u00e0n to\u00e0n) c\u0169ng kh\u00f4ng \u0111\u01b0\u1ee3c qu\u00e1 cao (chi ph\u00ed x\u1eed l\u00fd s\u1ebd kh\u00f4ng th\u1ec3 ch\u1ecbu n\u1ed5i). Nh\u01b0 \u0111\u00e3 tr\u00ecnh b\u00e0y \u1edf\u00a0hinh 4<\/a>, ng\u01b0\u1eddi ta quan s\u00e1t th\u1ea5y xu h\u01b0\u1edbng ng\u00e0y c\u00e0ng t\u0103ng c\u1ee7a s\u1ea3n l\u01b0\u1ee3ng quercitrin v\u00e0 TF, \u0111\u1ea1t gi\u00e1 tr\u1ecb cao nh\u1ea5t khi t\u1ef7 l\u1ec7 kho\u1ea3ng 10:1. M\u1ed9t l\u1eddi gi\u1ea3i th\u00edch c\u00f3 th\u1ec3 x\u1ea3y ra l\u00e0 vi\u1ec7c t\u0103ng t\u1ef7 l\u1ec7 dung m\u00f4i-v\u1eadt li\u1ec7u c\u00f3 th\u1ec3 l\u00e0m t\u0103ng kh\u1ea3 n\u0103ng khu\u1ebfch t\u00e1n c\u1ee7a dung m\u00f4i v\u00e0o t\u1ebf b\u00e0o v\u00e0 t\u0103ng c\u01b0\u1eddng gi\u1ea3i h\u1ea5p c\u00e1c h\u1ee3p ch\u1ea5t phenolic kh\u1ecfi t\u1ebf b\u00e0o.30<\/a>] Trong nghi\u00ean c\u1ee9u hi\u1ec7n t\u1ea1i, ng\u01b0\u1eddi ta th\u1ea5y s\u1ea3n l\u01b0\u1ee3ng quercitrin v\u00e0 TF gi\u1ea3m nh\u1eb9 khi gi\u00e1 tr\u1ecb t\u1ef7 l\u1ec7 cao h\u01a1n 10:1, \u0111i\u1ec1u n\u00e0y c\u00f3 th\u1ec3 l\u00e0 do t\u1ed5n th\u1ea5t l\u1edbn h\u01a1n trong qu\u00e1 tr\u00ecnh thu th\u1eadp s\u1ea3n ph\u1ea9m ho\u1eb7c v\u1eadn h\u00e0nh quy tr\u00ecnh. Do \u0111\u00f3, t\u1ef7 l\u1ec7 dung m\u00f4i-v\u1eadt li\u1ec7u \u0111\u01b0\u1ee3c \u0111\u1eb7t \u1edf m\u1ee9c 10:1 l\u00e0 m\u1ee9c trung b\u00ecnh cho RSM trong nghi\u00ean c\u1ee9u ti\u1ebfp theo.<\/p>\n

\n
\"An<\/div>\n
\n
hinh 4<\/a><\/div>\n
\n

\u1ea2nh h\u01b0\u1edfng c\u1ee7a t\u1ef7 l\u1ec7 dung m\u00f4i-v\u1eadt li\u1ec7u \u0111\u1ebfn hi\u1ec7u su\u1ea5t quercitrin v\u00e0 TF<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

\n

 <\/p>\n

\u1ea2nh h\u01b0\u1edfng c\u1ee7a k\u00edch th\u01b0\u1edbc h\u1ea1t<\/h4>\n

 <\/p>\n

K\u00edch th\u01b0\u1edbc h\u1ea1t l\u00e0 m\u1ed9t bi\u1ebfn quan tr\u1ecdng kh\u00e1c c\u1ea7n \u0111\u01b0\u1ee3c xem x\u00e9t. N\u00f3i chung, hi\u1ec7u su\u1ea5t chi\u1ebft t\u0103ng l\u00ean khi gi\u1ea3m k\u00edch th\u01b0\u1edbc h\u1ea1t [H\u00ecnh 5<\/a>]. Trong nghi\u00ean c\u1ee9u hi\u1ec7n t\u1ea1i, k\u00edch th\u01b0\u1edbc h\u1ea1t \u0111\u01b0\u1ee3c \u0111\u1eb7t \u1edf \u0111\u01b0\u1eddng k\u00ednh 0,45-0,9 mm.<\/p>\n

\n
\"An<\/div>\n
\n
H\u00ecnh 5<\/a><\/div>\n
\n

\u1ea2nh h\u01b0\u1edfng c\u1ee7a k\u00edch th\u01b0\u1edbc h\u1ea1t \u0111\u1ebfn hi\u1ec7u su\u1ea5t quercitrin v\u00e0 TF<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

\n

 <\/p>\n

\u1ea2nh h\u01b0\u1edfng c\u1ee7a th\u1eddi gian chi\u1ebft<\/h4>\n

 <\/p>\n

L\u1ef1a ch\u1ecdn th\u1eddi gian chi\u1ebft th\u00edch h\u1ee3p l\u00e0 b\u01b0\u1edbc cu\u1ed1i c\u00f9ng trong nghi\u00ean c\u1ee9u s\u01a1 b\u1ed9 c\u1ee7a ch\u00fang t\u00f4i. C\u00e1c \u0111\u1ed9 d\u00e0i chi\u1ebft kh\u00e1c nhau \u1edf 10, 20, 30, 40, 50 v\u00e0 60 ph\u00fat \u0111\u00e3 \u0111\u01b0\u1ee3c th\u1eed nghi\u1ec7m \u0111\u1ec3 ki\u1ec3m tra gi\u00e1 tr\u1ecb t\u1ed1i \u01b0u. K\u1ebft qu\u1ea3 cho th\u1ea5y hi\u1ec7u su\u1ea5t thu \u0111\u01b0\u1ee3c quercitrin v\u00e0 TF t\u0103ng l\u00ean khi k\u00e9o d\u00e0i th\u1eddi gian chi\u1ebft t\u1eeb 10 \u0111\u1ebfn 50 ph\u00fat [H\u00ecnh 6<\/a>]. \u1ede m\u1ee9c 50 ph\u00fat, s\u1ea3n l\u01b0\u1ee3ng quercitrin v\u00e0 TF \u0111\u1ea1t l\u01b0\u1ee3ng cao nh\u1ea5t. Do \u0111\u00f3, 40, 50 v\u00e0 60 ph\u00fat \u0111\u01b0\u1ee3c ch\u1ecdn l\u00e0m m\u1ee9c th\u1eddi gian chi\u1ebft th\u1ea5p h\u01a1n, trung b\u00ecnh v\u00e0 cao h\u01a1n.<\/p>\n

\n
\"An<\/div>\n
\n
H\u00ecnh 6<\/a><\/div>\n
\n

\u1ea2nh h\u01b0\u1edfng c\u1ee7a th\u1eddi gian chi\u1ebft \u0111\u1ebfn hi\u1ec7u su\u1ea5t quercitrin v\u00e0 TF<\/p>\n

 <\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

\n

T\u1ed1i \u01b0u h\u00f3a c\u00e1c th\u00f4ng s\u1ed1 tr\u00edch ly b\u1eb1ng RSM<\/h3>\n
\n

 <\/p>\n

K\u1ebft qu\u1ea3 h\u1ed3i quy tuy\u1ebfn t\u00ednh b\u1ed9i v\u00e0 ph\u00e2n t\u00edch m\u1ee9c \u0111\u1ed9 ph\u00f9 h\u1ee3p c\u1ee7a m\u00f4 h\u00ecnh \u0111\u01b0\u1ee3c trang b\u1ecb<\/h4>\n

 <\/p>\n

M\u1ed9t CCD xoay ba y\u1ebfu t\u1ed1 v\u00e0 ba m\u1ee9c bao g\u1ed3m 20 l\u1ea7n ch\u1ea1y th\u1eed nghi\u1ec7m \u0111\u00e3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng v\u1edbi 6 l\u1ea7n l\u1eb7p l\u1ea1i \u1edf \u0111i\u1ec3m trung t\u00e2m. \u1ea2nh h\u01b0\u1edfng c\u1ee7a s\u1ef1 bi\u1ebfn \u0111\u1ed5i kh\u00f4ng gi\u1ea3i th\u00edch \u0111\u01b0\u1ee3c \u0111\u01b0\u1ee3c gi\u1ea3m thi\u1ec3u b\u1eb1ng c\u00e1ch ng\u1eabu nhi\u00ean h\u00f3a th\u1ee9 t\u1ef1 th\u00ed nghi\u1ec7m. C\u00e1c bi\u1ebfn \u0111\u1ed9c l\u1eadp l\u00e0 th\u00e0nh ph\u1ea7n dung m\u00f4i (X1<\/sub>, %,\u00a0v\/v<\/em>, etanol\/n\u01b0\u1edbc), t\u1ef7 l\u1ec7 dung m\u00f4i-v\u1eadt li\u1ec7u (X2<\/sub>, ml\/g) v\u00e0 th\u1eddi gian chi\u1ebft (X3<\/sub>, ph\u00fat) v\u1edbi s\u1ea3n l\u01b0\u1ee3ng quercitrin v\u00e0 TF l\u00e0m ch\u1ea5t ch\u1ec9 th\u1ecb. K\u00edch th\u01b0\u1edbc h\u1ea1t c\u1ed1 \u0111\u1ecbnh (0,45-0,9 mm) \u0111\u00e3 \u0111\u01b0\u1ee3c ch\u1ecdn. S\u1ea3n l\u01b0\u1ee3ng quercitrin v\u00e0 TF c\u1ee7a t\u1ea5t c\u1ea3 c\u00e1c l\u1ea7n ch\u1ea1y \u0111\u01b0\u1ee3c th\u1ec3 hi\u1ec7n trong\u00a0B\u1ea3ng 1<\/a>. Nhi\u1ec1u h\u1ed3i quy tuy\u1ebfn t\u00ednh s\u1eed d\u1ee5ng m\u00f4 h\u00ecnh \u0111a th\u1ee9c b\u1eadc hai (Ph\u01b0\u01a1ng tr\u00ecnh (1)) \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n d\u1ef1a tr\u00ean c\u00e1c k\u1ebft qu\u1ea3 n\u00e0y.<\/p>\n

 <\/p>\n

\n

B\u1ea3ng 1<\/h3>\n
\n

C\u00e0i \u0111\u1eb7t thi\u1ebft k\u1ebf h\u1ed7n h\u1ee3p trung t\u00e2m c\u00f3 th\u1ec3 xoay \u1edf d\u1ea1ng ban \u0111\u1ea7u v\u00e0 \u0111\u01b0\u1ee3c m\u00e3 h\u00f3a c\u1ee7a c\u00e1c bi\u1ebfn \u0111\u1ed9c l\u1eadp (X1<\/sub>, X2<\/sub>, X3<\/sub>) v\u00e0 k\u1ebft qu\u1ea3 th\u1ef1c nghi\u1ec7m v\u1ec1 c\u00e1c bi\u1ebfn ph\u1ea3n \u1ee9ng, quercitrin v\u00e0 TF<\/p>\n<\/div>\n

\"An<\/div>\n<\/div>\n

ANOVA c\u1ee7a c\u00e1c bi\u1ebfn \u0111\u1ed9c l\u1eadp \u0111\u01b0\u1ee3c hi\u1ec3n th\u1ecb trong\u00a0ban 2<\/a>\u00a0ch\u1ec9 ra r\u1eb1ng c\u1ea3 ba bi\u1ebfn \u0111\u1ed9c l\u1eadp n\u00e0y \u0111\u1ec1u \u1ea3nh h\u01b0\u1edfng \u0111\u00e1ng k\u1ec3 \u0111\u1ebfn hi\u1ec7u su\u1ea5t chi\u1ebft (P<\/em>\u00a0< 0,05). C\u00e1c tham s\u1ed1 h\u1ed3i quy c\u1ee7a m\u00f4 h\u00ecnh b\u1eadc hai \u0111\u01b0\u1ee3c trang b\u1ecb v\u1edbi c\u00e1c h\u1ec7 s\u1ed1 x\u00e1c \u0111\u1ecbnh b\u1ed9i t\u01b0\u01a1ng \u1ee9ng (R<\/em>2<\/sup>) \u0111\u01b0\u1ee3c th\u1ec3 hi\u1ec7n \u1edf\u00a0b\u00e0n s\u1ed1 3<\/a>. \u0110\u00e3 \u0111\u1ea1t \u0111\u01b0\u1ee3c s\u1ef1 ph\u00f9 h\u1ee3p t\u1ed1t v\u00e0 s\u1ef1 thay \u0111\u1ed5i c\u1ee7a ph\u1ea3n \u1ee9ng \u0111\u00e3 \u0111\u01b0\u1ee3c gi\u1ea3i th\u00edch b\u1eb1ng m\u00f4 h\u00ecnh. Cao\u00a0R<\/em>2<\/sup>\u00a0c\u00e1c gi\u00e1 tr\u1ecb l\u1ea7n l\u01b0\u1ee3t l\u00e0 0, 94 v\u00e0 0, 96 cho quercitrin v\u00e0 TF, ng\u1ee5 \u00fd d\u1eef li\u1ec7u th\u1eed nghi\u1ec7m x\u00e1c nh\u1eadn t\u00ednh t\u01b0\u01a1ng th\u00edch v\u1edbi d\u1eef li\u1ec7u \u0111\u01b0\u1ee3c m\u00f4 h\u00ecnh d\u1ef1 \u0111o\u00e1n. Gi\u00e1 tr\u1ecb th\u1ea5p c\u1ee7a h\u1ec7 s\u1ed1 bi\u1ebfn thi\u00ean (1.92% \u0111\u1ed1i v\u1edbi quercitrin, 3.28% \u0111\u1ed1i v\u1edbi TF) cho th\u1ea5y k\u1ebft qu\u1ea3 thu \u0111\u01b0\u1ee3c t\u1eeb m\u00f4 h\u00ecnh \u0111\u01b0\u1ee3c trang b\u1ecb l\u00e0 \u0111\u00e1ng tin c\u1eady. H\u1ec7 s\u1ed1 x\u00e1c \u0111\u1ecbnh \u0111\u01b0\u1ee3c \u0111i\u1ec1u ch\u1ec9nh (R<\/em>2<\/sup>\u00a0Gi\u00e1 tr\u1ecb \u0110i\u1ec1u ch\u1ec9nh) s\u1ebd x\u00e2y d\u1ef1ng l\u1ea1i bi\u1ec3u th\u1ee9c v\u1edbi t\u1ea5t c\u1ea3 c\u00e1c thu\u1eadt ng\u1eef *c\u00f3 \u00fd ngh\u0129a \u0111\u01b0\u1ee3c bao g\u1ed3m v\u00e0 gi\u00e1 tr\u1ecb cao c\u1ee7a\u00a0R<\/em>2<\/sup>\u00a0T\u00ednh t\u1eeb. (R<\/em>2<\/sup>\u00a0T\u00ednh t\u1eeb. t\u01b0\u01a1ng \u1ee9ng l\u00e0 87%, 92%) h\u1ed7 tr\u1ee3 \u00fd ngh\u0129a c\u1ee7a m\u00f4 h\u00ecnh.<\/p>\n

 <\/p>\n

\n

ban 2<\/h3>\n
\n

Ph\u00e2n t\u00edch ph\u01b0\u01a1ng sai cho m\u00f4 h\u00ecnh b\u1eadc hai b\u1ec1 m\u1eb7t \u0111\u00e1p \u1ee9ng<\/p>\n<\/div>\n

\"An<\/div>\n
<\/div>\n<\/div>\n
\n

<\/h3>\n

b\u00e0n s\u1ed1 3<\/h3>\n
\n

H\u1ec7 s\u1ed1 h\u1ed3i quy c\u1ee7a m\u00f4 h\u00ecnh d\u1ef1 \u0111o\u00e1n b\u1eadc hai cho c\u00e1c bi\u1ebfn ph\u1ea3n \u1ee9ng, quercitrin v\u00e0 TF<\/p>\n<\/div>\n

\"An<\/div>\n<\/div>\n

Th\u1eed nghi\u1ec7m thi\u1ebfu ph\u00f9 h\u1ee3p \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 x\u00e1c minh t\u00ednh ph\u00f9 h\u1ee3p c\u1ee7a m\u00f4 h\u00ecnh. ANOVA cho th\u1eed nghi\u1ec7m thi\u1ebfu ph\u00f9 h\u1ee3p l\u00e0 kh\u00f4ng \u0111\u00e1ng k\u1ec3 \u0111\u1ed1i v\u1edbi s\u1ea3n l\u01b0\u1ee3ng quercitrin v\u00e0 TF (P<\/em>\u00a0> 0,05), cho th\u1ea5y m\u00f4 h\u00ecnh c\u00f3 th\u1ec3 ph\u00f9 h\u1ee3p \u0111\u1ea7y \u0111\u1ee7 v\u1edbi d\u1eef li\u1ec7u th\u1ef1c nghi\u1ec7m [B\u1ea3ng 4<\/a>].<\/p>\n

 <\/p>\n

\n

B\u1ea3ng 4<\/h3>\n
\n

Ph\u00e2n t\u00edch ph\u01b0\u01a1ng sai do thi\u1ebfu th\u1eed nghi\u1ec7m ph\u00f9 h\u1ee3p \u0111\u1ed1i v\u1edbi quercitrin v\u00e0 TF<\/p>\n<\/div>\n

\"An<\/div>\n<\/div>\n<\/div>\n
\n

 <\/p>\n

Ph\u00e2n t\u00edch b\u1ec1 m\u1eb7t ph\u1ea3n \u1ee9ng<\/h4>\n

 <\/p>\n

T\u1eeb; c\u00e1c m\u00f4 h\u00ecnh cho th\u1ea5y s\u1ef1 ph\u00f9 h\u1ee3p t\u1ed1t, c\u00e1c gi\u00e1 tr\u1ecb \u0111\u00e1p \u1ee9ng \u0111\u01b0\u1ee3c gi\u1ea3i th\u00edch \u0111\u1ea7y \u0111\u1ee7 b\u1eb1ng ph\u01b0\u01a1ng tr\u00ecnh h\u1ed3i quy. C\u00e1c \u0111\u01b0\u1eddng cong b\u1ec1 m\u1eb7t ph\u1ea3n \u1ee9ng \u0111\u01b0\u1ee3c v\u1ebd \u0111\u1ec3 th\u1ec3 hi\u1ec7n s\u1ef1 t\u01b0\u01a1ng t\u00e1c c\u1ee7a c\u00e1c bi\u1ebfn \u0111\u1ed9c l\u1eadp v\u00e0 \u0111\u1ec3 x\u00e1c \u0111\u1ecbnh gi\u00e1 tr\u1ecb t\u1ed1i \u01b0u c\u1ee7a t\u1eebng bi\u1ebfn \u0111\u1ed9c l\u1eadp \u0111\u1ec3 c\u00f3 ph\u1ea3n h\u1ed3i t\u1ed1i \u0111a. M\u1ed1i quan h\u1ec7 gi\u1eefa c\u00e1c bi\u1ebfn \u0111\u1ed9c l\u1eadp v\u00e0 ph\u1ee5 thu\u1ed9c \u0111\u01b0\u1ee3c minh h\u1ecda b\u1eb1ng c\u00e1c bi\u1ec3u \u0111\u1ed3 b\u1ec1 m\u1eb7t ph\u1ea3n \u1ee9ng ba chi\u1ec1u (3D) v\u00e0 c\u00e1c bi\u1ec3u \u0111\u1ed3 \u0111\u01b0\u1eddng vi\u1ec1n hai chi\u1ec1u (2D). B\u1ec1 m\u1eb7t \u0111\u00e1p \u1ee9ng 3D v\u00e0 c\u00e1c \u0111\u01b0\u1eddng \u0111\u1ed3ng m\u1ee9c 2D \u0111\u01b0\u1ee3c cung c\u1ea5p d\u01b0\u1edbi d\u1ea1ng bi\u1ec3u di\u1ec5n \u0111\u1ed3 h\u1ecda c\u1ee7a ph\u01b0\u01a1ng tr\u00ecnh h\u1ed3i quy [H\u00ecnh\u00a0\u200b[H\u00ecnh7<\/span>7<\/a>\u00a0V\u00e0\u00a0\u200bv\u00e08].<\/span>8<\/a>]. M\u1ed7i \u0111\u01b0\u1eddng cong bi\u1ec3u th\u1ecb v\u00f4 s\u1ed1 s\u1ef1 k\u1ebft h\u1ee3p c\u1ee7a hai bi\u1ebfn \u0111\u1ed9c l\u1eadp trong khi v\u1eabn gi\u1eef bi\u1ebfn \u0111\u1ed9c l\u1eadp c\u00f2n l\u1ea1i \u1edf \u0111i\u1ec3m d\u1eebng. B\u1eb1ng c\u00e1ch \u0111\u00f3, b\u1ec1 m\u1eb7t ph\u1ea3n h\u1ed3i 3D c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c h\u00ecnh dung d\u1ec5 d\u00e0ng.\u00a0H\u00ecnh 7a<\/a>\u00a0hi\u1ec3n th\u1ecb h\u00e0m b\u1ec1 m\u1eb7t ph\u1ea3n \u1ee9ng 3D \u0111\u01b0\u1ee3c ph\u00e1t tri\u1ec3n b\u1edfi m\u00f4 h\u00ecnh v\u1ec1 th\u00e0nh ph\u1ea7n dung m\u00f4i v\u00e0 t\u1ef7 l\u1ec7 dung m\u00f4i-v\u1eadt li\u1ec7u. Th\u00e0nh ph\u1ea7n dung m\u00f4i th\u1ec3 hi\u1ec7n t\u00e1c \u0111\u1ed9ng b\u1eadc hai l\u00ean ph\u1ea3n \u1ee9ng. H\u00e0m l\u01b0\u1ee3ng quercitrin \u0111\u1ea1t gi\u00e1 tr\u1ecb cao nh\u1ea5t khi s\u1eed d\u1ee5ng ethanol 65%. T\u01b0\u01a1ng t\u1ef1, t\u1ef7 l\u1ec7 dung m\u00f4i-v\u1eadt li\u1ec7u \u0111\u1ed3ng th\u1eddi th\u1ec3 hi\u1ec7n t\u00e1c d\u1ee5ng b\u1eadc hai \u0111\u1ed1i v\u1edbi quercitrin. K\u1ebft qu\u1ea3 \u1ea3nh h\u01b0\u1edfng c\u1ee7a th\u00e0nh ph\u1ea7n dung m\u00f4i v\u00e0 t\u1ef7 l\u1ec7 dung m\u00f4i \u2013 v\u1eadt li\u1ec7u \u0111\u1ebfn TF \u0111\u01b0\u1ee3c th\u1ec3 hi\u1ec7n \u1edf\u00a0H\u00ecnh 8a<\/a>. Th\u00e0nh ph\u1ea7n dung m\u00f4i th\u1ec3 hi\u1ec7n hi\u1ec7u \u1ee9ng b\u1eadc hai \u0111\u1ed1i v\u1edbi ph\u1ea3n \u1ee9ng mang l\u1ea1i hi\u1ec7u su\u1ea5t t\u1ed1i \u0111a gi\u1eefa etanol 60% v\u00e0 70%. T\u01b0\u01a1ng t\u1ef1, khi c\u1ed1 \u0111\u1ecbnh th\u00e0nh ph\u1ea7n dung m\u00f4i b\u1eb1ng c\u00e1ch t\u0103ng t\u1ef7 l\u1ec7 dung m\u00f4i-v\u1eadt li\u1ec7u, TF t\u0103ng d\u1ea7n v\u00e0 \u0111\u1ea1t gi\u00e1 tr\u1ecb cao nh\u1ea5t khi t\u1ef7 l\u1ec7 dung m\u00f4i-v\u1eadt li\u1ec7u kho\u1ea3ng 10:1.<\/p>\n

\n
\"An<\/div>\n
\n
H\u00ecnh 7<\/a><\/div>\n
\n

\u0110\u1ed3 th\u1ecb b\u1ec1 m\u1eb7t ph\u1ea3n \u1ee9ng (a, c v\u00e0 e) v\u00e0 \u0111\u1ed3 th\u1ecb \u0111\u01b0\u1eddng vi\u1ec1n (b, d v\u00e0 f) th\u1ec3 hi\u1ec7n \u1ea3nh h\u01b0\u1edfng c\u1ee7a th\u00e0nh ph\u1ea7n dung m\u00f4i (X1), t\u1ef7 l\u1ec7 v\u1eadt li\u1ec7u dung m\u00f4i (X2) v\u00e0 th\u1eddi gian chi\u1ebft (X3) \u0111\u1ebfn hi\u1ec7u su\u1ea5t quercitrin<\/p>\n<\/div>\n<\/div>\n<\/div>\n

\n
\"An<\/div>\n
\n
H\u00ecnh 8<\/a><\/div>\n
<\/div>\n
\n

\u0110\u1ed3 th\u1ecb b\u1ec1 m\u1eb7t ph\u1ea3n \u1ee9ng (a, c v\u00e0 e) v\u00e0 \u0111\u1ed3 th\u1ecb \u0111\u01b0\u1eddng vi\u1ec1n (b, d v\u00e0 f) th\u1ec3 hi\u1ec7n \u1ea3nh h\u01b0\u1edfng c\u1ee7a th\u00e0nh ph\u1ea7n dung m\u00f4i (X1), t\u1ef7 l\u1ec7 v\u1eadt li\u1ec7u dung m\u00f4i (X2) v\u00e0 th\u1eddi gian chi\u1ebft (X3) \u0111\u1ebfn hi\u1ec7u su\u1ea5t TF<\/p>\n<\/div>\n<\/div>\n<\/div>\n

\u1ea2nh h\u01b0\u1edfng c\u1ee7a c\u00e1c bi\u1ebfn \u0111\u1ed9c l\u1eadp th\u00e0nh ph\u1ea7n dung m\u00f4i v\u00e0 th\u1eddi gian chi\u1ebft \u0111\u1ebfn gi\u00e1 tr\u1ecb ph\u1ea3n \u1ee9ng c\u1ee7a hi\u1ec7u su\u1ea5t quercitrin v\u00e0 TF \u0111\u01b0\u1ee3c th\u1ec3 hi\u1ec7n trong H\u00ecnh\u00a0\u200bH\u00ecnh7c,<\/span>7c<\/a>,\u00a0\u200b,7 ng\u00e0y,<\/span>7 ng\u00e0y<\/a>,\u00a0\u200b,8c,<\/span>8c<\/a>,\u00a0\u200b,8 ng\u00e0y,<\/span>8 ng\u00e0y<\/a>, t\u01b0\u01a1ng \u1ee9ng. C\u00f3 th\u1ec3 d\u1ec5 d\u00e0ng hi\u1ec3u \u0111\u01b0\u1ee3c m\u1ed1i quan h\u1ec7 t\u01b0\u01a1ng t\u00e1c gi\u1eefa hai bi\u1ebfn \u0111\u1ed9c l\u1eadp b\u1eb1ng c\u00e1ch ki\u1ec3m tra c\u00e1c \u0111\u01b0\u1eddng \u0111\u1ed3ng m\u1ee9c \u0111\u01b0\u1ee3c t\u1ea1o b\u1eb1ng c\u00e1ch gi\u1eef cho bi\u1ebfn \u0111\u1ed9c l\u1eadp kh\u00e1c, t\u1ef7 l\u1ec7 dung m\u00f4i-v\u1eadt li\u1ec7u, kh\u00f4ng \u0111\u1ed5i t\u1ea1i \u0111i\u1ec3m d\u1eebng [H\u00ecnh\u00a0\u200b[H\u00ecnh7d<\/span>7 ng\u00e0y<\/a>\u00a0V\u00e0\u00a0\u200bv\u00e08d].<\/span>8 ng\u00e0y<\/a>]. S\u1ef1 gia t\u0103ng s\u1ea3n l\u01b0\u1ee3ng quercitrin v\u00e0 TF \u0111\u1ea1t \u0111\u01b0\u1ee3c \u0111\u00e1ng k\u1ec3 khi t\u0103ng th\u00e0nh ph\u1ea7n dung m\u00f4i v\u00e0 th\u1eddi gian chi\u1ebft. Tuy nhi\u00ean, hi\u1ec7u su\u1ea5t quercitrin v\u00e0 TF kh\u00f4ng c\u00f2n t\u0103ng khi c\u00e1c bi\u1ebfn \u0111\u1ed9c l\u1eadp v\u01b0\u1ee3t qu\u00e1 gi\u00e1 tr\u1ecb nh\u1ea5t \u0111\u1ecbnh. S\u1ef1 t\u01b0\u01a1ng t\u00e1c gi\u1eefa th\u00e0nh ph\u1ea7n dung m\u00f4i v\u00e0 th\u1eddi gian chi\u1ebft kh\u00f4ng c\u00f3 \u00fd ngh\u0129a th\u1ed1ng k\u00ea trong vi\u1ec7c \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn hi\u1ec7u su\u1ea5t quercitrin, nh\u01b0ng n\u00f3 c\u00f3 \u00fd ngh\u0129a (P<\/em>\u00a0< 0,01) \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn vi\u1ec7c chi\u1ebft xu\u1ea5t TF.<\/p>\n

S\u1ed1 li\u1ec7u\u00a0\u200bH\u00ecnh7e<\/span>7e<\/a>\u00a0V\u00e0\u00a0\u200band8e<\/span>8e<\/a>\u00a0\u0111\u00e3 ch\u1ee9ng minh \u1ea3nh h\u01b0\u1edfng c\u1ee7a t\u1ef7 l\u1ec7 dung m\u00f4i-v\u1eadt li\u1ec7u v\u00e0 th\u1eddi gian chi\u1ebft \u0111\u1ebfn hi\u1ec7u su\u1ea5t quercitrin v\u00e0 TF. S\u1ef1 gia t\u0103ng hi\u1ec7u su\u1ea5t quercitrin v\u00e0 TF \u0111\u01b0\u1ee3c quan s\u00e1t th\u1ea5y c\u00f9ng v\u1edbi s\u1ef1 gia t\u0103ng th\u1eddi gian chi\u1ebft v\u00e0 t\u1ef7 l\u1ec7 dung m\u00f4i-v\u1eadt li\u1ec7u, \u0111i\u1ec1u n\u00e0y cho th\u1ea5y r\u1eb1ng hi\u1ec7u su\u1ea5t chi\u1ebft c\u1ee7a quercitrin v\u00e0 TF t\u1ef7 l\u1ec7 thu\u1eadn v\u1edbi th\u1eddi gian chi\u1ebft v\u00e0 t\u1ef7 l\u1ec7 dung m\u00f4i-v\u1eadt li\u1ec7u. S\u1ef1 t\u01b0\u01a1ng t\u00e1c c\u1ee7a hai bi\u1ebfn \u0111\u1ed9c l\u1eadp trong qu\u00e1 tr\u00ecnh chi\u1ebft xu\u1ea5t quercitrin v\u00e0 TF t\u1eeb Herba Polygoni Capitati kh\u00f4ng c\u00f3 \u00fd ngh\u0129a th\u1ed1ng k\u00ea.<\/p>\n

 <\/p>\n<\/div>\n<\/div>\n

\n

Th\u00ed nghi\u1ec7m x\u00e1c minh<\/h3>\n

 <\/p>\n

\u0110\u1ec3 x\u00e1c minh kh\u1ea3 n\u0103ng d\u1ef1 \u0111o\u00e1n c\u1ee7a m\u00f4 h\u00ecnh, c\u00e1c bi\u1ebfn ph\u1ea3n \u1ee9ng t\u1ed1i \u01b0u \u0111\u00e3 \u0111\u01b0\u1ee3c ki\u1ec3m tra trong c\u00e1c \u0111i\u1ec1u ki\u1ec7n t\u1ed1i \u01b0u h\u00f3a b\u1eaft ngu\u1ed3n t\u1eeb ph\u00e2n t\u00edch \u0111\u01b0\u1eddng v\u00e2n c\u1ee7a RSM. K\u1ebft qu\u1ea3 l\u00e0, c\u00e1c gi\u00e1 tr\u1ecb quan s\u00e1t \u0111\u01b0\u1ee3c c\u00f3 th\u1ec3 \u0111\u1ea1t \u0111\u01b0\u1ee3c m\u1ed9t c\u00e1ch th\u1ecfa \u0111\u00e1ng trong kho\u1ea3ng tin c\u1eady 95% c\u1ee7a c\u00e1c gi\u00e1 tr\u1ecb d\u1ef1 \u0111o\u00e1n t\u1eeb m\u00f4 h\u00ecnh [B\u1ea3ng 5<\/a>].<\/p>\n

 <\/p>\n

\n

B\u1ea3ng 5<\/h3>\n
\n

So s\u00e1nh gi\u1eefa gi\u00e1 tr\u1ecb d\u1ef1 \u0111o\u00e1n v\u00e0 gi\u00e1 tr\u1ecb quan s\u00e1t \u0111\u01b0\u1ee3c c\u1ee7a c\u00e1c bi\u1ebfn ph\u1ea3n \u1ee9ng, quercitrin v\u00e0 TF<\/p>\n<\/div>\n

\"An<\/div>\n<\/div>\n<\/div>\n<\/div>\n
\n
<\/div>\n
\u0110i \u0111\u1ebfn:<\/a><\/div>\n

K\u1ebeT LU\u1eacN<\/h2>\n

 <\/p>\n

B\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng ph\u01b0\u01a1ng ph\u00e1p ti\u1ebfp c\u1eadn m\u1ed9t y\u1ebfu t\u1ed1 t\u1ea1i m\u1ed9t th\u1eddi \u0111i\u1ec3m k\u1ebft h\u1ee3p v\u1edbi CCD, c\u00e1c th\u00f4ng s\u1ed1 chi\u1ebft c\u00f3 s\u1ef1 h\u1ed7 tr\u1ee3 c\u1ee7a si\u00eau \u00e2m, bao g\u1ed3m th\u00e0nh ph\u1ea7n dung m\u00f4i, t\u1ef7 l\u1ec7 dung m\u00f4i-v\u1eadt li\u1ec7u v\u00e0 th\u1eddi gian chi\u1ebft \u0111\u00e3 \u0111\u01b0\u1ee3c t\u1ed1i \u01b0u h\u00f3a \u0111\u1ec3 chi\u1ebft quercitrin v\u00e0 TF t\u1eeb Herba Polygoni Capitati. K\u1ebft qu\u1ea3 cho th\u1ea5y m\u00f4 h\u00ecnh \u0111a th\u1ee9c b\u1eadc hai \u0111\u00e3 m\u00f4 t\u1ea3 th\u1ecfa \u0111\u00e1ng d\u1eef li\u1ec7u tr\u00edch xu\u1ea5t. C\u00e1c \u0111i\u1ec1u ki\u1ec7n t\u1ed1i \u01b0u nh\u01b0 sau: Ph\u01b0\u01a1ng ph\u00e1p chi\u1ebft, chi\u1ebft si\u00eau \u00e2m; dung m\u00f4i, c\u00f3 th\u1ec3 l\u00e0m tr\u00f2n \u1edf m\u1ee9c 65% dung d\u1ecbch ethanol; k\u00edch th\u01b0\u1edbc h\u1ea1t 0,45-0,9 mm; t\u1ef7 l\u1ec7 dung m\u00f4i-v\u1eadt li\u1ec7u, l\u00e0m tr\u00f2n \u0111\u1ebfn 10:1; th\u1eddi gian chi\u1ebft, l\u00e0m tr\u00f2n \u0111\u1ebfn 54 ph\u00fat. Nghi\u00ean c\u1ee9u n\u00e0y c\u00f3 th\u1ec3 cung c\u1ea5p th\u00f4ng tin c\u01a1 b\u1ea3n v\u00e0 c\u00e1i nh\u00ecn s\u00e2u s\u1eafc c\u00f3 gi\u00e1 tr\u1ecb v\u1ec1 quy tr\u00ecnh khai th\u00e1c c\u00f4ng nghi\u1ec7p c\u1ee7a Herba Polygoni Capitati.<\/p>\n

 <\/p>\n<\/div>\n

\n
\u0110i \u0111\u1ebfn:<\/a><\/div>\n

S\u1ef0 NH\u00ccN NH\u1eacN<\/h2>\n

 <\/p>\n

\n

C\u00e1c t\u00e1c gi\u1ea3 xin ch\u00e2n th\u00e0nh c\u1ea3m \u01a1n T\u1eadp \u0111o\u00e0n D\u01b0\u1ee3c ph\u1ea9m Qu\u00fd Ch\u00e2u Warmen \u0111\u00e3 cung c\u1ea5p nguy\u00ean li\u1ec7u th\u00f4 v\u00e0 h\u1ed7 tr\u1ee3 t\u00e0i ch\u00ednh t\u1eeb Ch\u01b0\u01a1ng tr\u00ecnh \u0111\u1eb7c bi\u1ec7t Khoa h\u1ecdc v\u00e0 C\u00f4ng ngh\u1ec7 l\u1edbn c\u1ee7a t\u1ec9nh Qu\u00fd Ch\u00e2u (s\u1ed1 2011 6019-02), Nh\u00f3m T\u00e0i n\u0103ng \u0110\u1ed5i m\u1edbi Khoa h\u1ecdc v\u00e0 C\u00f4ng ngh\u1ec7 T\u1ec9nh Qu\u00fd Ch\u00e2u v\u1ec1 Ph\u00e2n t\u00edch D\u01b0\u1ee3c ph\u1ea9m (S\u1ed1 . 2011 4008), Ch\u01b0\u01a1ng tr\u00ecnh \u0111\u1eb7c bi\u1ec7t c\u1ee7a t\u1ec9nh Qu\u00fd Ch\u00e2u v\u1ec1 Nghi\u00ean c\u1ee9u v\u00e0 Ph\u00e1t tri\u1ec3n ng\u00e0nh Khoa h\u1ecdc v\u00e0 C\u00f4ng ngh\u1ec7 v\u1ec1 Hi\u1ec7n \u0111\u1ea1i h\u00f3a Y h\u1ecdc c\u1ed5 truy\u1ec1n (S\u1ed1 ZY 2011 3013), Ph\u00f2ng th\u00ed nghi\u1ec7m tr\u1ecdng \u0111i\u1ec3m \u0111\u1eb7c tr\u01b0ng v\u1ec1 ti\u00eau chu\u1ea9n h\u00f3a thu\u1ed1c c\u1ed5 truy\u1ec1n Trung Qu\u1ed1c v\u00e0 thu\u1ed1c qu\u1ed1c gia v\u00e0 Ph\u00f2ng th\u00ed nghi\u1ec7m tr\u1ecdng \u0111i\u1ec3m \u0111\u1eb7c tr\u01b0ng c\u1ee7a Qu\u00fd Ch\u00e2u S\u1edf gi\u00e1o d\u1ee5c t\u1ec9nh. (s\u1ed1 KY 2012 005).<\/p>\n

 <\/p>\n<\/div>\n<\/div>\n

\n
\u0110i \u0111\u1ebfn:<\/a><\/div>\n

Ch\u00fa th\u00edch cu\u1ed1i trang<\/h2>\n
\n

\n

Ngu\u1ed3n h\u1ed7 tr\u1ee3:<\/strong>\u00a0Kh\u00f4ng<\/p>\n

Xung \u0111\u1ed9t l\u1ee3i \u00edch:<\/strong>\u00a0Kh\u00f4ng c\u00f3 tuy\u00ean b\u1ed1.<\/p>\n

 <\/p>\n<\/div>\n<\/div>\n

\n
\u0110i \u0111\u1ebfn:<\/a><\/div>\n

NG\u01af\u1edcI GI\u1edaI THI\u1ec6U<\/h2>\n

 <\/p>\n

\n
1.\u00a0Ren G, Chang F, Lu S, Zhong H, Zhang G. Nghi\u00ean c\u1ee9u d\u01b0\u1ee3c l\u00fd c\u1ee7a\u00a0\u0110a gi\u00e1c \u0111\u1ea7u ng\u01b0\u1eddi<\/em>\u00a0Buch-Ham.ex D. Don.\u00a0Chin J Chin Mater Med.\u00a0<\/span>1995;2<\/span>: 107\u20139. 28. [PubMed<\/a>]\u00a0[H\u1ecdc gi\u1ea3 Google<\/a>]<\/span><\/span><\/div>\n
2.\u00a0Li ML, Liang B, Tang JW, Xie Y, Zuo AP, Du F. Nghi\u00ean c\u1ee9u d\u01b0\u1ee3c l\u1ef1c h\u1ecdc v\u1ec1 h\u1ea1t Relinqing M\u00f4 h\u00ecnh vi\u00eam b\u1ec3 th\u1eadn do vi khu\u1ea9n d\u1ea7u \u1edf chu\u1ed9t.\u00a0Chin J Chin Mater Med.\u00a0<\/span>2006;2<\/span>:153\u20135. [PubMed<\/a>]\u00a0[H\u1ecdc gi\u1ea3 Google<\/a>]<\/span><\/span><\/div>\n
3.\u00a0Cheng YS, Wang JY, Hao JJ. T\u00e1c d\u1ee5ng c\u1ee7a Tolterodine li\u00ean quan \u0111\u1ebfn h\u1ea1t Relinqing trong \u0111i\u1ec1u tr\u1ecb b\u00e0ng quang ho\u1ea1t \u0111\u1ed9ng qu\u00e1 m\u1ee9c.\u00a0Ph\u00f2ng kh\u00e1m ch\u1eefa c\u1eb1m.\u00a0<\/span>2012;4<\/span>:500\u20131.\u00a0[H\u1ecdc gi\u1ea3 Google<\/a>]<\/span><\/span><\/div>\n
4.\u00a0Pereira MA, Grubbs CJ, Barnes LH, Li H, Olson GR, Eto I, et al. T\u00e1c d\u1ee5ng c\u1ee7a c\u00e1c ch\u1ea5t phytochemical, curcumin v\u00e0 quercetin \u0111\u1ed1i v\u1edbi b\u1ec7nh ung th\u01b0 ru\u1ed9t k\u1ebft do azoxymethane g\u00e2y ra v\u00e0 ung th\u01b0 v\u00fa do 7,12-dimethylbenz [a] anthracene g\u00e2y ra \u1edf chu\u1ed9t.\u00a0Ch\u1ea5t g\u00e2y ung th\u01b0.\u00a0<\/span>1996;6<\/span>:1305\u201311. [PubMed<\/a>]\u00a0[H\u1ecdc gi\u1ea3 Google<\/a>]<\/span><\/span><\/div>\n
5.\u00a0Caltagirone S, Ranelletti FO, Rinelli A, Maggiano N, Colasante A, Musiani P, v\u00e0 c\u1ed9ng s\u1ef1. T\u01b0\u01a1ng t\u00e1c v\u1edbi c\u00e1c v\u1ecb tr\u00ed g\u1eafn estrogen lo\u1ea1i II v\u00e0 ho\u1ea1t \u0111\u1ed9ng ch\u1ed1ng t\u0103ng sinh c\u1ee7a tamoxifen v\u00e0 quercetin trong b\u1ec7nh ung th\u01b0 ph\u1ed5i kh\u00f4ng ph\u1ea3i t\u1ebf b\u00e0o nh\u1ecf \u1edf ng\u01b0\u1eddi.\u00a0Am J Respir Cell Mol Biol.\u00a0<\/span>1997;1<\/span>:51\u20139. [PubMed<\/a>]\u00a0[H\u1ecdc gi\u1ea3 Google<\/a>]<\/span><\/span><\/div>\n
6.\u00a0Aviram M, Fuhrman B. Polyphenolic flavonoid \u1ee9c ch\u1ebf qu\u00e1 tr\u00ecnh oxy h\u00f3a LDL qua trung gian \u0111\u1ea1i th\u1ef1c b\u00e0o v\u00e0 l\u00e0m gi\u1ea3m qu\u00e1 tr\u00ecnh x\u01a1 v\u1eefa \u0111\u1ed9ng m\u1ea1ch.\u00a0X\u01a1 v\u1eefa \u0111\u1ed9ng m\u1ea1ch.\u00a0<\/span>1998;137<\/span>(B\u1ed5 sung):S45\u201350. [PubMed<\/a>]\u00a0[H\u1ecdc gi\u1ea3 Google<\/a>]<\/span><\/span><\/div>\n
7.\u00a0Yan XL, Li CQ, Liu YX, Chang X, Kang WY. Ho\u1ea1t \u0111\u1ed9ng ch\u1ed1ng oxy h\u00f3a c\u1ee7a polynum capitatum.\u00a0Chin Pharm.\u00a0<\/span>2010;39<\/span>:3659\u201361.\u00a0[H\u1ecdc gi\u1ea3 Google<\/a>]<\/span><\/span><\/div>\n
8.\u00a0Siess MH, Leclerc J, Canivenc-Lavier MC, Rat P, Suschetet M. T\u00e1c d\u1ee5ng kh\u00f4ng \u0111\u1ed3ng nh\u1ea5t c\u1ee7a flavonoid t\u1ef1 nhi\u00ean \u0111\u1ed1i v\u1edbi ho\u1ea1t \u0111\u1ed9ng monooxygenase trong microsome gan ng\u01b0\u1eddi v\u00e0 chu\u1ed9t.\u00a0D\u01b0\u1ee3c ph\u1ea9m \u1ee9ng d\u1ee5ng Toxicol.\u00a0<\/span>1995;1<\/span>:73\u20138. [PubMed<\/a>]\u00a0[H\u1ecdc gi\u1ea3 Google<\/a>]<\/span><\/span><\/div>\n
9.\u00a0Agullo G, Gamet-Payrastre L, Manenti S, Viala C, R\u00e9m\u00e9sy C, Chap H, et al. M\u1ed1i quan h\u1ec7 gi\u1eefa c\u1ea5u tr\u00fac flavonoid v\u00e0 s\u1ef1 \u1ee9c ch\u1ebf phosphatidylinositol 3-kinase: So s\u00e1nh v\u1edbi s\u1ef1 \u1ee9c ch\u1ebf tyrosine kinase v\u00e0 protein kinase C.\u00a0D\u01b0\u1ee3c ph\u1ea9m sinh h\u1ecdc.\u00a0<\/span>1997;11<\/span>:1649\u201357. [PubMed<\/a>]\u00a0[H\u1ecdc gi\u1ea3 Google<\/a>]<\/span><\/span><\/div>\n
10.\u00a0Conseil G, Baubichon-Cortay H, Dayan G, Jault JM, Barron D, Di Pietro A. Flavonoid: M\u1ed9t lo\u1ea1i ch\u1ea5t \u0111i\u1ec1u bi\u1ebfn c\u00f3 t\u01b0\u01a1ng t\u00e1c hai ch\u1ee9c n\u0103ng t\u1ea1i c\u00e1c v\u1ecb tr\u00ed g\u1eafn ATP- v\u00e0 steroid ph\u1ee5 tr\u00ean P-glycoprotein c\u1ee7a chu\u1ed9t.\u00a0Proc Natl Acad Sci US A.\u00a0<\/span>1998;17<\/span>:9831\u20136.\u00a0[B\u00e0i vi\u1ebft mi\u1ec5n ph\u00ed c\u1ee7a PMC<\/a>]<\/span>\u00a0[PubMed<\/a>]\u00a0[H\u1ecdc gi\u1ea3 Google<\/a>]<\/span><\/span><\/div>\n
11.\u00a0Hertog MG, Kromhout D, Aravanis C, Blackburn H, Buzina R, Fidanza F, v\u00e0 nh\u1eefng ng\u01b0\u1eddi kh\u00e1c. Nghi\u00ean c\u1ee9u v\u1ec1 vi\u1ec7c s\u1eed d\u1ee5ng flavonoid v\u00e0 nguy c\u01a1 l\u00e2u d\u00e0i m\u1eafc b\u1ec7nh tim m\u1ea1ch v\u00e0nh v\u00e0 ung th\u01b0 \u1edf b\u1ea3y qu\u1ed1c gia.\u00a0Arch Intern Med.\u00a0<\/span>1995;4<\/span>:381\u20136. [PubMed<\/a>]\u00a0[H\u1ecdc gi\u1ea3 Google<\/a>]<\/span><\/span><\/div>\n
12.\u00a0Liyana-Pathirana C, Shahidi F. T\u1ed1i \u01b0u h\u00f3a vi\u1ec7c chi\u1ebft xu\u1ea5t c\u00e1c h\u1ee3p ch\u1ea5t phenolic t\u1eeb l\u00faa m\u00ec b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p b\u1ec1 m\u1eb7t ph\u1ea3n \u1ee9ng.\u00a0H\u00f3a ch\u1ea5t th\u1ef1c ph\u1ea9m.\u00a0<\/span>2005;1<\/span>:47\u201356.\u00a0[H\u1ecdc gi\u1ea3 Google<\/a>]<\/span><\/span><\/div>\n
13.\u00a0Li WL, Zheng HC, Bukuru J, De Kimpe N. C\u00e1c lo\u1ea1i thu\u1ed1c t\u1ef1 nhi\u00ean \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng trong h\u1ec7 th\u1ed1ng y h\u1ecdc c\u1ed5 truy\u1ec1n Trung Qu\u1ed1c \u0111\u1ec3 \u0111i\u1ec1u tr\u1ecb b\u1ec7nh \u0111\u00e1i th\u00e1o \u0111\u01b0\u1eddng.\u00a0J Ethnopharmacol.\u00a0<\/span>2004;1<\/span>: 1\u201321. [PubMed<\/a>]\u00a0[H\u1ecdc gi\u1ea3 Google<\/a>]<\/span><\/span><\/div>\n
14.\u00a0Normile D. Y h\u1ecdc ch\u00e2u \u00c1. Di\u1ec7n m\u1ea1o m\u1edbi c\u1ee7a y h\u1ecdc c\u1ed5 truy\u1ec1n Trung Qu\u1ed1c.\u00a0Khoa h\u1ecdc.\u00a0<\/span>2003;5604<\/span>: 188\u201390. [PubMed<\/a>]\u00a0[H\u1ecdc gi\u1ea3 Google<\/a>]<\/span><\/span><\/div>\n
15.\u00a0Chi\u1ebft xu\u1ea5t DeFeudis FV, Drieu K. Ginkgo biloba (EGb 761) v\u00e0 ch\u1ee9c n\u0103ng CNS: Nghi\u00ean c\u1ee9u c\u01a1 b\u1ea3n v\u00e0 \u1ee9ng d\u1ee5ng l\u00e2m s\u00e0ng.\u00a0M\u1ee5c ti\u00eau thu\u1ed1c Curr.\u00a0<\/span>2000;1<\/span>: 25\u201358. [PubMed<\/a>]\u00a0[H\u1ecdc gi\u1ea3 Google<\/a>]<\/span><\/span><\/div>\n
16.\u00a0Rowland I. Dinh d\u01b0\u1ee1ng t\u1ed1i \u01b0u: Ch\u1ea5t x\u01a1 v\u00e0 ch\u1ea5t phytochemical.\u00a0Proc Nutr Soc.\u00a0<\/span>1999;02<\/span>:415\u20139. [PubMed<\/a>]\u00a0[H\u1ecdc gi\u1ea3 Google<\/a>]<\/span><\/span><\/div>\n
17.\u00a0Yang BB, Feng R, Wang WC, Zhang LY, Ye XM, Wang Y, et al. Ph\u00e2n t\u00edch \u0111\u1ecbnh l\u01b0\u1ee3ng ba th\u00e0nh ph\u1ea7n ho\u1ea1t t\u00ednh trong th\u1ea3o m\u1ed9c v\u00f9ng Miao, Polygonum capitatum b\u1eb1ng HPLC\/DAD\/MS.\u00a0Chin J Pharm H\u1eadu M\u00f4n.\u00a0<\/span>2008;11<\/span>:1793\u20136.\u00a0[H\u1ecdc gi\u1ea3 Google<\/a>]<\/span><\/span><\/div>\n
18.\u00a0Xu J, Zhang YP, Cheng SX. Nghi\u00ean c\u1ee9u chi\u1ebft xu\u1ea5t c\u00f3 h\u1ed7 tr\u1ee3 vi s\u00f3ng t\u1eeb\u00a0Polygonum capitatum Buch.-Ham. b\u00e1n t\u1ea1i<\/em>\u00a0D.\u00a0Don J Med Pharm Chin Minor.\u00a0<\/span>2012;8<\/span>:76\u20137.\u00a0[H\u1ecdc gi\u1ea3 Google<\/a>]<\/span><\/span><\/div>\n
19.\u00a0Zhao XC, Bai H, Wang YS, Liu AQ, Li GQ. Quy tr\u00ecnh t\u00e1ch chi\u1ebft flavonoid t\u1ed5ng s\u1ed1 c\u1ee7a c\u00e2y \u0111a gi\u00e1c.\u00a0Lishizhen Med Mater Med Res.\u00a0<\/span>2009;11<\/span>:2815\u20136.\u00a0[H\u1ecdc gi\u1ea3 Google<\/a>]<\/span><\/span><\/div>\n
20.\u00a0Silva EM, Rogez H, Larondelle Y. T\u1ed1i \u01b0u h\u00f3a qu\u00e1 tr\u00ecnh chi\u1ebft xu\u1ea5t phenolics t\u1eeb l\u00e1 Inga edulis b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p b\u1ec1 m\u1eb7t ph\u1ea3n \u1ee9ng.\u00a0C\u00f4ng ngh\u1ec7 Purif th\u00e1ng 9.\u00a0<\/span>2007;3<\/span>:381\u20137.\u00a0[H\u1ecdc gi\u1ea3 Google<\/a>]<\/span><\/span><\/div>\n
21.\u00a0Tian S, Chu X, Gong H, Ma X, Zhang F. Thi\u1ebft k\u1ebf th\u1eed nghi\u1ec7m tr\u1ef1c giao \u0111\u1ec3 t\u1ed1i \u01b0u h\u00f3a qu\u00e1 tr\u00ecnh chi\u1ebft polysaccharide t\u1eeb Paeonia sinjiangensis KY Pan.\u00a0T\u1ea1p ch\u00ed d\u01b0\u1ee3c ph\u1ea9m.\u00a0<\/span>2011;25<\/span>:4\u20138.\u00a0[B\u00e0i vi\u1ebft mi\u1ec5n ph\u00ed c\u1ee7a PMC<\/a>]<\/span>\u00a0[PubMed<\/a>]\u00a0[H\u1ecdc gi\u1ea3 Google<\/a>]<\/span><\/span><\/div>\n
22.\u00a0Arslan-Alaton I, Tureli G, Olmez-Hanci T. X\u1eed l\u00fd n\u01b0\u1edbc th\u1ea3i s\u1ea3n xu\u1ea5t thu\u1ed1c nhu\u1ed9m azo b\u1eb1ng quy tr\u00ecnh oxy h\u00f3a n\u00e2ng cao gi\u1ed1ng Photo-Fenton: T\u1ed1i \u01b0u h\u00f3a b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p ph\u1ea3n \u1ee9ng b\u1ec1 m\u1eb7t.\u00a0J Photochem Photobiol M\u1ed9t Chem.\u00a0<\/span>2009;2\u20133<\/span>:142\u201353.\u00a0[H\u1ecdc gi\u1ea3 Google<\/a>]<\/span><\/span><\/div>\n
23.\u00a0Tang X, Yan L, Gao J, Ge H, Yang H, Lin N. T\u1ed1i \u01b0u h\u00f3a quy tr\u00ecnh chi\u1ebft v\u00e0 kh\u1ea3o s\u00e1t t\u00e1c d\u1ee5ng ch\u1ed1ng oxy h\u00f3a c\u1ee7a polysaccharide t\u1eeb r\u1ec5 c\u00e2y\u00a0Limonium sinense<\/em>\u00a0Kuntze.\u00a0T\u1ea1p ch\u00ed d\u01b0\u1ee3c ph\u1ea9m.\u00a0<\/span>2011;27<\/span>: 186\u201392.\u00a0[B\u00e0i vi\u1ebft mi\u1ec5n ph\u00ed c\u1ee7a PMC<\/a>]<\/span>\u00a0[PubMed<\/a>]\u00a0[H\u1ecdc gi\u1ea3 Google<\/a>]<\/span><\/span><\/div>\n
24.\u00a0Ba\u015f D, Boyac\u0131 \u0130H. M\u00f4 h\u00ecnh h\u00f3a v\u00e0 t\u1ed1i \u01b0u h\u00f3a I: Kh\u1ea3 n\u0103ng s\u1eed d\u1ee5ng ph\u01b0\u01a1ng ph\u00e1p b\u1ec1 m\u1eb7t \u0111\u00e1p \u1ee9ng.\u00a0J Food Eng.\u00a0<\/span>2007;3<\/span>:836\u201345.\u00a0[H\u1ecdc gi\u1ea3 Google<\/a>]<\/span><\/span><\/div>\n
25.\u00a0Bahorun T, Luximon-Ramma A, Crozier A, Aruoma OI. T\u1ed5ng h\u00e0m l\u01b0\u1ee3ng phenol, flavonoid, proanthocyanidin v\u00e0 vitamin C v\u00e0 ho\u1ea1t \u0111\u1ed9ng ch\u1ed1ng oxy h\u00f3a c\u1ee7a rau Mauriti.\u00a0J Sci Food Agric.\u00a0<\/span>2004;12<\/span>:1553\u201361.\u00a0[H\u1ecdc gi\u1ea3 Google<\/a>]<\/span><\/span><\/div>\n
26.\u00a0Lin JY, Tang CY. X\u00e1c \u0111\u1ecbnh t\u1ed5ng h\u00e0m l\u01b0\u1ee3ng phenolic v\u00e0 flavonoid trong c\u00e1c lo\u1ea1i tr\u00e1i c\u00e2y v\u00e0 rau qu\u1ea3 \u0111\u01b0\u1ee3c ch\u1ecdn, c\u0169ng nh\u01b0 t\u00e1c d\u1ee5ng k\u00edch th\u00edch c\u1ee7a ch\u00fang \u0111\u1ed1i v\u1edbi s\u1ef1 t\u0103ng sinh t\u1ebf b\u00e0o l\u00e1ch c\u1ee7a chu\u1ed9t.\u00a0H\u00f3a ch\u1ea5t th\u1ef1c ph\u1ea9m.\u00a0<\/span>2007;1<\/span>: 140\u20137.\u00a0[H\u1ecdc gi\u1ea3 Google<\/a>]<\/span><\/span><\/div>\n
27.\u00a0Meda A, Lamien CE, Romito M, Millogo J, Nacoulma OG. X\u00e1c \u0111\u1ecbnh t\u1ed5ng h\u00e0m l\u01b0\u1ee3ng phenolic, flavonoid v\u00e0 proline trong m\u1eadt ong Burkina Fasan c\u0169ng nh\u01b0 ho\u1ea1t t\u00ednh lo\u1ea1i b\u1ecf g\u1ed1c t\u1ef1 do c\u1ee7a ch\u00fang.\u00a0H\u00f3a ch\u1ea5t th\u1ef1c ph\u1ea9m.\u00a0<\/span>2005;3<\/span>:571\u20137.\u00a0[H\u1ecdc gi\u1ea3 Google<\/a>]<\/span><\/span><\/div>\n
28.\u00a0Xie Z, Zhao Y, Chen P, Jing P, Yue J, Yu L. Ph\u00e2n t\u00edch d\u1ea5u v\u00e2n tay s\u1eafc k\u00fd v\u00e0 c\u00e1c th\u00e0nh ph\u1ea7n rutin v\u00e0 quercetin trong c\u00e1c m\u1eabu l\u00e1 v\u00e0 to\u00e0n b\u1ed9 c\u00e2y c\u1ee7a Di- v\u00e0 t\u1ee9 b\u1ed9i gynostemma pentaphyllum.\u00a0J Agric Th\u1ef1c ph\u1ea9m Chem.\u00a0<\/span>2011;7<\/span>:3042\u20139. [PubMed<\/a>]\u00a0[H\u1ecdc gi\u1ea3 Google<\/a>]<\/span><\/span><\/div>\n
29.\u00a0Volpi N. \u1ee8ng d\u1ee5ng \u0111i\u1ec7n di mao qu\u1ea3n hi\u1ec7u su\u1ea5t cao v\u00e0o qu\u00e1 tr\u00ecnh tinh ch\u1ebf\u00a0Escherichia coli<\/em>\u00a0K4 polysacarit.\u00a0J S\u1eafc k\u00fd B.\u00a0<\/span>2004;2<\/span>:253\u20136. [PubMed<\/a>]\u00a0[H\u1ecdc gi\u1ea3 Google<\/a>]<\/span><\/span><\/div>\n
30.\u00a0Ray B. Polysaccharides t\u1eeb Enteromorpha Compressa: \u0110\u1eb7c \u0111i\u1ec3m ph\u00e2n l\u1eadp, tinh ch\u1ebf v\u00e0 c\u1ea5u tr\u00fac.\u00a0Polyme cacbonat.\u00a0<\/span>2006;3<\/span>:408\u201316.\u00a0[H\u1ecdc gi\u1ea3 Google<\/a>]<\/span><\/span><\/div>\n<\/div>\n<\/div>","protected":false},"excerpt":{"rendered":"

Herba Polygoni Capitati, the aerial part or whole plant of Polygonum capitatum Buch.-Ham. ex D. Don, has been used as one of the Traditional Chinese Medicines (TCMs) for a long time in China, especially in some ethnic minority regions.<\/p>","protected":false},"author":1,"featured_media":5353,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[9],"tags":[],"class_list":["post-5097","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-supplements"],"_links":{"self":[{"href":"https:\/\/directcm.com\/vi\/wp-json\/wp\/v2\/posts\/5097","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/directcm.com\/vi\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/directcm.com\/vi\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/directcm.com\/vi\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/directcm.com\/vi\/wp-json\/wp\/v2\/comments?post=5097"}],"version-history":[{"count":1,"href":"https:\/\/directcm.com\/vi\/wp-json\/wp\/v2\/posts\/5097\/revisions"}],"predecessor-version":[{"id":5380,"href":"https:\/\/directcm.com\/vi\/wp-json\/wp\/v2\/posts\/5097\/revisions\/5380"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/directcm.com\/vi\/wp-json\/wp\/v2\/media\/5353"}],"wp:attachment":[{"href":"https:\/\/directcm.com\/vi\/wp-json\/wp\/v2\/media?parent=5097"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/directcm.com\/vi\/wp-json\/wp\/v2\/categories?post=5097"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/directcm.com\/vi\/wp-json\/wp\/v2\/tags?post=5097"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}